| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Garcia, Brady E (10)
|
NITTSC |
43.64 |
40.67 |
-2.97 |
| 2 |
Attuwaybi, Hamza B (10)
|
NIVELO |
41.27 |
41.52 |
0.25 |
| 3 |
Lee, Aidan R (8)
|
BORC |
43.09 |
41.73 |
-1.36 |
| 4 |
Moran, Aiden T (9)
|
NICLSC |
32.65 |
43.70 |
11.05 |
| 5 |
Anderson, Max (9)
|
NIVELO |
44.74 |
44.58 |
-0.16 |
| 6 |
Coen, Mitchell R (9)
|
NISTAR |
45.78 |
46.21 |
0.43 |
| 7 |
Hediger, Kobe K (10)
|
NIUN-E |
49.73 |
46.55 |
-3.18 |
| 8 |
Chen, Ethan (9)
|
BORC |
47.89 |
46.59 |
-1.30 |
| 9 |
Young-Mandiak, Atticus F (10)
|
NISTAR |
47.15 |
47.40 |
0.25 |
| 10 |
Matteson, Cyrus L (10)
|
NISTAR |
52.46 |
47.94 |
-4.52 |
| 11 |
King, Emmett M (9)
|
NITTSC |
47.22 |
48.40 |
1.18 |
| 12 |
Shepler, Jack (10)
|
NIVELO |
47.13 |
48.85 |
1.72 |
| 13 |
Elshamy, Yassine (9)
|
BORC |
49.69 |
49.03 |
-0.66 |
| 14 |
Wang, Michael (9)
|
NIVELO |
48.86 |
49.12 |
0.26 |
| 15 |
Moldenhauer, Thomas C (9)
|
NIEAST |
53.17 |
50.20 |
-2.97 |
| 16 |
Reimann, Nathan S (9)
|
NISTAR |
54.61 |
50.24 |
-4.37 |
| 17 |
Rogers, Joseph T (9)
|
NISTAR |
|
50.37 |
- |
| 18 |
Labib, Yassin (10)
|
BORC |
50.05 |
50.56 |
0.51 |
| 19 |
Kneeland, Noah J (9)
|
NIVELO |
55.71 |
52.29 |
-3.42 |
| 20 |
Voit, Nicholas (10)
|
NIAMTS |
|
52.90 |
- |
| 21 |
Fisher, Simon D (10)
|
NISTAR |
53.93 |
54.20 |
0.27 |
| 22 |
DellaMea, Nicholas A (9)
|
NIVELO |
56.69 |
55.60 |
-1.09 |
| 23 |
Nienhaus, Matthew (9)
|
NISHAQ |
54.64 |
57.16 |
2.52 |
| 24 |
DellaMea, Marco V (9)
|
NIVELO |
58.43 |
57.67 |
-0.76 |
| 25 |
Sauls, Clarke W (9)
|
NICLSC |
|
58.58 |
- |
| 26 |
Pirigyi, Ethan J (10)
|
NISTAR |
58.50 |
58.71 |
0.21 |
| 27 |
Olsen, Owen T (10)
|
NIAMTS |
|
1:01.02 |
- |
| 28 |
Crowther, Finn L (10)
|
NIVELO |
1:03.61 |
1:01.79 |
-1.82 |
| 29 |
Kendall, Alex M (10)
|
NITTSC |
1:06.50 |
1:05.16 |
-1.34 |
| 30 |
Walter, Jacob P (10)
|
NITTSC |
1:03.09 |
1:08.36 |
5.27 |
| 31 |
Chelpinski, Alexander (10)
|
NIVELO |
|
1:16.38 |
- |
|
Reukauf, Nathan A (9)
|
NISTAR |
1:08.26 |
DQ |
- |
|
Rudnicki, William S (10)
|
NITWST |
|
NS |
- |