| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Umansky, Katarina R (8)
|
NIBAAC |
41.89 |
41.99 |
0.10 |
| 2 |
Anderson, Lennon L (8)
|
NIVELO |
44.23 |
42.44 |
-1.79 |
| 3 |
Adams, Mallory B (8)
|
NISHAQ |
46.10 |
47.59 |
1.49 |
| 4 |
Loeffler, Madeline G (8)
|
NIBAAC |
48.68 |
48.49 |
-0.19 |
| 5 |
Marcyan, Reese J (8)
|
NITTSC |
48.20 |
48.81 |
0.61 |
| 6 |
Rivers, Keira E (8)
|
NIWWSC |
54.85 |
53.46 |
-1.39 |
| 7 |
Natemeier, Margo K (8)
|
NIVICT |
54.12 |
57.16 |
3.04 |
| 8 |
Brownell, Grace M (7)
|
NISTAR |
1:05.16 |
59.29 |
-5.87 |
| 9 |
Gaia, Theresa M (8)
|
NISHAQ |
59.11 |
59.46 |
0.35 |
| 10 |
Adams, Elliana H (8)
|
NISTAR |
56.85 |
59.51 |
2.66 |
| 11 |
Nudell, Lucy G (8)
|
NITTSC |
|
1:00.56 |
- |
| 12 |
Strittmatter, Anastasia R (8)
|
NISTAR |
1:08.07 |
1:02.46 |
-5.61 |
| 13 |
Garigen, Molly G (7)
|
NIWEBS |
1:10.87 |
1:03.17 |
-7.70 |
| 14 |
Martinez Reese, Elida M (8)
|
NISTAR |
|
1:19.19 |
- |
|
Harter, Violet E (7)
|
NIRAYS |
|
DQ |
- |
|
Baker, Ava (5)
|
NISHAQ |
1:39.80 |
DQ |
- |
|
Muffoletto, Elise R (7)
|
NITTSC |
|
DQ |
- |