| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Reilly, Bridget F (9)
|
NISTAR |
55.42 |
49.39 |
-6.03 |
| 2 |
Puzanovova, Veronika A (9)
|
NIBAAC |
56.42 |
50.04 |
-6.38 |
| 3 |
Wands, Lucille M (9)
|
NIVELO |
49.66 |
50.21 |
0.55 |
| 4 |
Golden, Lauren B (9)
|
NIBAAC |
52.59 |
51.18 |
-1.41 |
| 5 |
Rasmusson, Henrietta E (9)
|
NIUN-C |
52.66 |
53.59 |
0.93 |
| 6 |
Horn, Erin E (9)
|
NIEAST |
55.09 |
53.71 |
-1.38 |
| 7 |
Burnham, Madeline (9)
|
NIVELO |
|
53.76 |
- |
| 8 |
Tunkey, Mila R (9)
|
NIEAST |
1:08.67 |
54.00 |
-14.67 |
| 9 |
Zinno, Isabella G (9)
|
NICLSC |
|
56.75 |
- |
| 10 |
Attuwaybi, Eman (9)
|
NIVELO |
1:00.34 |
58.83 |
-1.51 |
| 11 |
Oneill, Meghan R (9)
|
NICLSC |
|
59.50 |
- |
| 12 |
Seelig, Jordan L (9)
|
NIEAST |
1:04.08 |
1:01.94 |
-2.14 |
| 13 |
Washington, Ashanti M (9)
|
NISTAR |
|
1:04.96 |
- |
| 14 |
Bialy, Stella D (9)
|
NISTAR |
|
1:07.58 |
- |
|
Alessi, Luciana M (9)
|
NIBAAC |
54.75 |
DQ |
- |
|
King, Izzy M (9)
|
NIEAST |
55.22 |
NS |
- |
|
Rogalski, Siena A (9)
|
NIBAAC |
1:05.36 |
NS |
- |