| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Anderson, Madden (10)
|
NIVELO |
42.69 |
41.56 |
-1.13 |
| 2 |
Wamsley, Sarah E (10)
|
NIVELO |
43.97 |
46.30 |
2.33 |
| 3 |
Reukauf, Anna R (10)
|
NISTAR |
46.41 |
46.51 |
0.10 |
| 4 |
Reif, Rydell (10)
|
NISHAQ |
47.78 |
46.96 |
-0.82 |
| 5 |
Wands, Lucille M (10)
|
NIVELO |
48.19 |
48.01 |
-0.18 |
| 6 |
Marchese, Cara E (10)
|
NIBAAC |
52.47 |
51.04 |
-1.43 |
| 7 |
Umansky, Isabella C (10)
|
NIBAAC |
53.29 |
51.76 |
-1.53 |
| 8 |
Strittmatter, Amelia C (10)
|
NISTAR |
56.49 |
53.13 |
-3.36 |
| 9 |
Tothero, Madeleine S (10)
|
NISTAR |
52.75 |
53.16 |
0.41 |
| 10 |
Barrera-Hagner, Ananya S (10)
|
NIEAST |
53.93 |
53.45 |
-0.48 |
| 11 |
Horn, Erin E (10)
|
NIEAST |
53.68 |
54.07 |
0.39 |
| 12 |
Winfield, Susannah K (10)
|
NIEAST |
|
57.38 |
- |
| 13 |
Crozier, Sydney (10)
|
NIVELO |
56.74 |
58.19 |
1.45 |
| 14 |
Arcadi, Kayla A (10)
|
NIVELO |
1:01.10 |
1:06.98 |
5.88 |
|
Balcom, Lucy W (10)
|
NIBAAC |
59.38 |
NS |
- |
|
Morey, Celia R (10)
|
NIBAAC |
52.10 |
NS |
- |