| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Reilly, Fiona K (11)
|
NISTAR |
38.57 |
38.75 |
0.18 |
| 2 |
Chung, Emily (11)
|
NIBAAC |
39.98 |
38.98 |
-1.00 |
| 3 |
Griffin, Zoey O (11)
|
NICLSC |
41.37 |
39.73 |
-1.64 |
| 4 |
Adamczyk, Gabrielle J (11)
|
NICLSC |
40.83 |
40.58 |
-0.25 |
| 5 |
McKinney, Madison N (11)
|
NICLSC |
40.09 |
40.61 |
0.52 |
| 6 |
Moldenhauer, Paige A (11)
|
NIEAST |
41.79 |
41.43 |
-0.36 |
| 7 |
Philbrick, Mylie E (11)
|
NIVELO |
43.98 |
42.99 |
-0.99 |
| 8 |
Gachette, Marisa B (11)
|
NIVELO |
43.22 |
43.35 |
0.13 |
| 9 |
Brassard, Addison G (11)
|
NISTAR |
46.31 |
44.53 |
-1.78 |
| 10 |
Vogt, Grace A (11)
|
NISTAR |
43.57 |
44.60 |
1.03 |
| 11 |
Battistoni, Grace L (11)
|
NIVELO |
45.33 |
45.42 |
0.09 |
| 12 |
Barillari, Emma (11)
|
NIVELO |
46.27 |
45.62 |
-0.65 |
| 13 |
Harrison, Ella C (11)
|
NICLSC |
45.24 |
45.77 |
0.53 |
| 14 |
Swiencicki, Nora M (11)
|
NICLSC |
|
46.19 |
- |
| 15 |
Seelig, Jenna N (11)
|
NICLSC |
46.37 |
46.46 |
0.09 |
| 16 |
Gentile, Olivia (11)
|
NIVELO |
46.80 |
46.79 |
-0.01 |
| 17 |
Adams, Daphne J (11)
|
NIBAAC |
52.16 |
48.81 |
-3.35 |
| 18 |
Kaufman, Bridget K (11)
|
NICLSC |
50.07 |
48.83 |
-1.24 |
| 19 |
Weisenburg, Brylee M (11)
|
NISTAR |
47.58 |
49.17 |
1.59 |
| 20 |
Booth, Sophia A (11)
|
NISTAR |
|
50.84 |
- |
| 21 |
Renzoni, Olivia R (11)
|
NISTAR |
1:06.74 |
52.25 |
-14.49 |
| 22 |
Nuara, Gabrielle S (11)
|
NICLSC |
50.41 |
52.52 |
2.11 |
| 23 |
Shepperd, Sloane A (11)
|
NIEAST |
1:03.67 |
52.76 |
-10.91 |
| 24 |
Cotter, Keara L (11)
|
NISTAR |
50.55 |
53.66 |
3.11 |
| 25 |
Schaefer, Grace I (11)
|
NIEAST |
55.59 |
55.49 |
-0.10 |
| 26 |
Meade, Juliana E (11)
|
NISTAR |
55.10 |
55.95 |
0.85 |
| 27 |
Rivera, Grace M (11)
|
NISTAR |
|
1:03.55 |
- |
|
Lawson, Addison C (11)
|
NISTAR |
|
DQ |
- |
|
Betzig, Brooklynn K (11)
|
NISTAR |
|
NS |
- |