| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Cartenuto, Emily M (11)
|
NICLSC |
43.78 |
37.92 |
-5.86 |
| 2 |
Reukauf, Anna R (11)
|
NISTAR |
40.15 |
38.81 |
-1.34 |
| 3 |
Wamsley, Sarah E (11)
|
NIVELO |
41.37 |
40.78 |
-0.59 |
| 4 |
Casler, Hannah R (11)
|
NIFAST |
42.00 |
41.56 |
-0.44 |
| 5 |
Wands, Lucille M (11)
|
NIVELO |
42.30 |
41.73 |
-0.57 |
| 6 |
Morey, Celia R (11)
|
NIBAAC |
44.38 |
41.96 |
-2.42 |
| 7 |
Zinno, Gianna M (11)
|
NICLSC |
44.32 |
42.20 |
-2.12 |
| 8 |
Marchese, Cara E (11)
|
NIBAAC |
51.04 |
43.01 |
-8.03 |
| 9 |
Urbanek, Addison E (11)
|
NICLSC |
45.75 |
43.11 |
-2.64 |
| 10 |
Lu, Ella (11)
|
NIVELO |
|
43.53 |
- |
| 11 |
King, Skylar L (11)
|
NIEAST |
43.06 |
44.14 |
1.08 |
| 12 |
Oneill, Meghan R (11)
|
NICLSC |
45.72 |
44.54 |
-1.18 |
| 13 |
Arroyo, Lucia R (11)
|
NICLSC |
47.97 |
44.99 |
-2.98 |
| 14 |
McCully, Hannah M (11)
|
NISHAQ |
51.13 |
46.08 |
-5.05 |
| 15 |
Tothero, Madeleine S (11)
|
NISTAR |
48.85 |
46.82 |
-2.03 |
| 16 |
Kalbfus, Taylor R (11)
|
NIFAST |
46.46 |
47.33 |
0.87 |
| 17 |
Umansky, Isabella C (11)
|
NIBAAC |
48.60 |
47.72 |
-0.88 |
| 18 |
Whitley-Grassi, Ava E (11)
|
NISTAR |
51.70 |
47.78 |
-3.92 |
| 19 |
Przybysz, Tara (11)
|
NIBAAC |
50.74 |
48.11 |
-2.63 |
| 20 |
Kreher, Laura G (11)
|
NICLSC |
|
48.57 |
- |
| 21 |
Horn, Erin E (11)
|
NIEAST |
51.61 |
49.44 |
-2.17 |
| 22 |
Goldman, Bailee M (11)
|
NICLSC |
50.70 |
52.10 |
1.40 |
| 23 |
Kauffman, Faith M (11)
|
NISTAR |
51.69 |
52.79 |
1.10 |
| 24 |
Haines, Kaylee N (11)
|
NISTAR |
54.08 |
54.06 |
-0.02 |
| 25 |
Waid, Alexandra H (11)
|
NISTAR |
54.57 |
55.32 |
0.75 |
| 26 |
Hennessey, Caitlin G (11)
|
NIVELO |
|
56.33 |
- |
| 27 |
Golab, Brooke M (11)
|
NISTAR |
1:04.24 |
1:04.82 |
0.58 |
|
James, Harper L (11)
|
NISTAR |
|
DQ |
- |
|
Kimball, Meredith S (11)
|
NISTAR |
1:06.04 |
NS |
- |
|
Gawron, Samantha G (11)
|
NICLSC |
45.06 |
NS |
- |