| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Keller, Mira (11)
|
NIVELO |
41.02 |
38.81 |
-2.21 |
| 2 |
Reilly, Fiona K (11)
|
NISTAR |
38.57 |
38.93 |
0.36 |
| 3 |
Adamczyk, Gabrielle J (11)
|
NICLSC |
49.60 |
40.83 |
-8.77 |
| 4 |
Wands, Amelia G (11)
|
NIVELO |
40.09 |
41.36 |
1.27 |
| 5 |
Moldenhauer, Paige A (11)
|
NIEAST |
43.52 |
41.79 |
-1.73 |
| 6 |
McKinney, Madison N (11)
|
NICLSC |
40.09 |
42.00 |
1.91 |
| 7 |
Vogt, Grace A (11)
|
NISTAR |
46.97 |
43.57 |
-3.40 |
| 8 |
Mintz, Vanessa M (11)
|
NICLSC |
43.40 |
44.49 |
1.09 |
| 9 |
Mitchell, Gwenyth E (11)
|
NIEMAC |
43.74 |
44.64 |
0.90 |
| 10 |
Harrison, Ella C (11)
|
NICLSC |
47.70 |
45.24 |
-2.46 |
| 11 |
Battistoni, Grace L (11)
|
NIVELO |
45.94 |
45.33 |
-0.61 |
| 12 |
Brassard, Addison G (11)
|
NISTAR |
49.70 |
46.31 |
-3.39 |
| 13 |
Seelig, Jenna N (11)
|
NICLSC |
48.48 |
46.37 |
-2.11 |
| 14 |
Gentile, Olivia (11)
|
NIVELO |
47.47 |
48.03 |
0.56 |
| 15 |
Boehringer, Maya L (11)
|
NICLSC |
47.93 |
48.14 |
0.21 |
| 15 |
Meade, Juliana E (11)
|
NISTAR |
1:01.58 |
55.10 |
-6.48 |
| 16 |
Nuara, Gabrielle S (11)
|
NICLSC |
55.19 |
50.41 |
-4.78 |
| 17 |
Cotter, Keara L (11)
|
NISTAR |
55.07 |
50.55 |
-4.52 |
| 18 |
Humphries, Hayden S (11)
|
NICLSC |
53.65 |
51.11 |
-2.54 |
| 19 |
Schaefer, Grace I (11)
|
NIEAST |
59.64 |
55.59 |
-4.05 |
|
Sciolino, Gianna R (11)
|
NICLSC |
|
DQ |
- |
|
Booth, Sophia A (11)
|
NISTAR |
|
DQ |
- |
|
Rivera, Grace M (11)
|
NISTAR |
|
DQ |
- |