| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Leighton, Evelyn S (12)
|
NISTAR |
34.71 |
35.97 |
1.26 |
| 2 |
Berdysiak, Elizabeth C (12)
|
NICLSC |
39.99 |
37.68 |
-2.31 |
| 3 |
Pirigyi, Sophia J (12)
|
NISTAR |
40.47 |
39.00 |
-1.47 |
| 4 |
Wands, Amelia G (12)
|
NIVELO |
40.09 |
39.47 |
-0.62 |
| 5 |
Domster, Madeline N (12)
|
NISTAR |
38.59 |
39.84 |
1.25 |
| 6 |
White-Thomson, Sierra N (12)
|
NISHAQ |
44.39 |
40.84 |
-3.55 |
| 7 |
Gauld, Alison M (12)
|
NISHAQ |
43.44 |
41.41 |
-2.03 |
| 8 |
Brown, Haley C (12)
|
NIVELO |
42.82 |
41.61 |
-1.21 |
| 9 |
McDonnell, Amelia R (12)
|
NISTAR |
41.97 |
41.82 |
-0.15 |
| 10 |
Lin, Isabella E (12)
|
NIVELO |
41.47 |
41.91 |
0.44 |
| 11 |
Fosberg, Ella G (12)
|
NISTAR |
44.55 |
43.34 |
-1.21 |
| 12 |
Klose, Shayna (12)
|
NIBAAC |
43.80 |
43.37 |
-0.43 |
| 13 |
Colder, Amelia W (12)
|
NISTAR |
42.61 |
43.99 |
1.38 |
| 14 |
Tothero, Katherine M (12)
|
NISTAR |
44.25 |
44.12 |
-0.13 |
| 15 |
Morelli, Emily G (12)
|
NICLSC |
45.84 |
46.31 |
0.47 |
| 16 |
Chelpinski, Angelica J (12)
|
NIVELO |
48.17 |
47.83 |
-0.34 |
| 17 |
Eisenmann, Abigail E (12)
|
NISTAR |
50.33 |
48.07 |
-2.26 |
| 18 |
Nadarajan, Gouripriya (12)
|
NIVELO |
48.37 |
48.61 |
0.24 |
| 19 |
Delille, Beatrice F (12)
|
NIVELO |
49.33 |
50.72 |
1.39 |
| 20 |
Humphries, Hayden S (12)
|
NICLSC |
51.11 |
51.73 |
0.62 |
|
Tesluk, Elizabeth D (12)
|
NISTAR |
36.10 |
NS |
- |
|
Pike, Olivia R (12)
|
NISTAR |
42.64 |
NS |
- |
|
Kreinheder, Sophie A (12)
|
NISTAR |
51.10 |
NS |
- |