| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Bashor, Allie E (16)
|
NISTAR |
2:32.32 |
2:30.39 |
-1.93 |
| 2 |
Noecker, Elle A (13)
|
NITWST |
2:37.50 |
2:32.57 |
-4.93 |
| 3 |
Webster, Laryssa G (15)
|
NIBRAD |
2:34.46 |
2:32.79 |
-1.67 |
| 4 |
Fritsch, Natalie A (14)
|
NITTSC |
2:35.85 |
2:35.41 |
-0.44 |
| 5 |
Keller, Mira (13)
|
NIVELO |
2:37.58 |
2:40.34 |
2.76 |
| 6 |
Chambers, Noa J (13)
|
NEATST |
2:53.06 |
2:40.73 |
-12.33 |
| 7 |
McDonnell, Lillian (18)
|
NISTAR |
2:31.84 |
2:41.59 |
9.75 |
| 8 |
Hewett, Molly K (18)
|
NISTAR |
2:41.13 |
2:43.40 |
2.27 |
| 9 |
El Ghissassi, Ritaj (14)
|
NIUN-0 |
2:41.37 |
2:43.59 |
2.22 |
| 10 |
Yohan, Tessa E (16)
|
NEATST |
2:50.88 |
2:44.11 |
-6.77 |
| 11 |
Studebaker, Gabrielle W (18)
|
NISTAR |
2:48.32 |
2:45.24 |
-3.08 |
| 12 |
Adamczyk, Gabrielle J (13)
|
NICLSC |
2:36.32 |
2:46.41 |
10.09 |
| 13 |
Morgus, Michaela T (18)
|
NISTAR |
|
2:47.12 |
- |
| 14 |
Moore, Kathleen E (14)
|
NIUN-D |
2:46.76 |
2:48.76 |
2.00 |
| 15 |
Mintz, Vanessa M (13)
|
NICLSC |
3:00.14 |
2:59.88 |
-0.26 |
| 16 |
Kassirer, Natalie G (13)
|
NITWST |
3:01.82 |
3:00.53 |
-1.29 |
| 17 |
McDonnell, Amelia R (14)
|
NISTAR |
2:57.45 |
3:00.87 |
3.42 |
| 18 |
Humby, Jessica L (15)
|
NISTAR |
3:06.08 |
3:05.28 |
-0.80 |
| 19 |
Acosta McKenzie, Vianka Y (17)
|
NEATST |
3:00.49 |
3:07.80 |
7.31 |
| 20 |
Griffin, Haley A (13)
|
NITTSC |
3:08.26 |
3:08.59 |
0.33 |
| 21 |
Blake, Elizabeth C (17)
|
NISTAR |
3:10.20 |
3:10.66 |
0.46 |
| 22 |
Beutner, Ella R (13)
|
NIVICT |
|
3:22.67 |
- |
| 23 |
Xu, Emily (16)
|
NIVELO |
|
3:25.29 |
- |
| 24 |
Wands, Amelia G (13)
|
NIVELO |
|
3:31.14 |
- |
| 25 |
Rietz, Tarin (15)
|
NIVELO |
3:52.81 |
3:41.39 |
-11.42 |
| 26 |
Lin, Isabella E (13)
|
NIVELO |
|
3:44.66 |
- |
| 27 |
Selig, Alexis J (13)
|
NEATST |
|
3:54.87 |
- |