| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wang, Bethany B (7)
|
NIKRKN |
36.57 |
36.58 |
0.01 |
| 2 |
Umansky, Katarina R (8)
|
NIBAAC |
39.62 |
39.53 |
-0.09 |
| 3 |
Loehfelm, Madelyn G (8)
|
NITWST |
44.06 |
39.99 |
-4.07 |
| 4 |
Attuwaybi, Hibah (8)
|
NIVELO |
42.60 |
40.02 |
-2.58 |
| 5 |
Van Slyke, Juliana C (8)
|
AMPEAQ |
44.30 |
40.61 |
-3.69 |
| 6 |
Adams, Mallory B (8)
|
NISHAQ |
44.36 |
40.91 |
-3.45 |
| 7 |
Anderson, Lennon (8)
|
NIVELO |
41.51 |
41.46 |
-0.05 |
| 8 |
Marcyan, Reese J (8)
|
NITTSC |
43.26 |
44.25 |
0.99 |
| 9 |
Bruzgul, Vaughn S (8)
|
NITWST |
42.49 |
44.69 |
2.20 |
| 10 |
Gaia, Theresa M (7)
|
NISHAQ |
59.92 |
54.02 |
-5.90 |
| 11 |
Vetter-Higgins, Mackenzie J (8)
|
NITTSC |
|
1:01.29 |
- |
| 12 |
Seplaki, Elise M (8)
|
NIVELO |
|
1:03.83 |
- |
| 13 |
Strittmatter, Anastasia R (8)
|
NISTAR |
|
1:05.97 |
- |
| 14 |
Willis, Adelaide R (8)
|
NISHAQ |
1:18.19 |
1:10.81 |
-7.38 |