| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
White, Emily G (12)
|
MANRG |
39.20 |
34.41 |
-4.79 |
| 2 |
Haralambous, Alexandria S (12)
|
MANRG |
41.43 |
35.37 |
-6.06 |
| 3 |
Kouser, Kaitlyn M (11)
|
MAUN B |
34.90 |
35.64 |
0.74 |
| 4 |
Montuori, Ava R (11)
|
MAWAVE |
36.86 |
36.23 |
-0.63 |
| 5 |
Luetke, Jordan (12)
|
MAUN B |
|
36.77 |
- |
| 6 |
Ammon, Isabella R (11)
|
MAFSA |
36.92 |
36.81 |
-0.11 |
| 7 |
Donahoe, Cheyenne M (11)
|
MANRG |
38.99 |
40.19 |
1.20 |
| 8 |
Jaskiewicz, Kaitlyn M (11)
|
MANRG |
40.91 |
40.31 |
-0.60 |
| 9 |
Christianson, Leah A (11)
|
MAWAVE |
43.06 |
40.92 |
-2.14 |
| 10 |
Montvydas, Sophia R (11)
|
MANRG |
41.21 |
41.28 |
0.07 |
| 11 |
Lin, Ava A (11)
|
MAFSA |
44.15 |
42.64 |
-1.51 |
| 12 |
Pappas, LucaRose N (11)
|
MAFSA |
52.51 |
43.88 |
-8.63 |