| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Tarolli, Vincent M (15)
|
NICSCI |
23.62 |
23.95 |
0.33 |
| 2 |
Thomas, Joshua D (17)
|
NIOLSC |
22.82 |
24.41 |
1.59 |
| 3 |
Scharoun, Keegan N (15)
|
NICSCI |
24.53 |
25.29 |
0.76 |
| 4 |
Barrington, Matthew W (15)
|
NICSCI |
29.15 |
27.21 |
-1.94 |
| 5 |
Drotar, Zach (13)
|
NISKAN |
27.45 |
28.27 |
0.82 |
| 6 |
Denkenberger, Quinten M (13)
|
NIOLSC |
27.90 |
28.77 |
0.87 |
| 7 |
Sledziona, Jaco (14)
|
NIOLSC |
32.54 |
32.17 |
-0.37 |
| 8 |
Winter, Brendan M (10)
|
NIWGSC |
38.38 |
37.44 |
-0.94 |
| 9 |
Milliman, Lathan (9)
|
NIWGSC |
43.73 |
42.42 |
-1.31 |
| 10 |
Krul, Landon C (10)
|
NIWGSC |
42.08 |
42.99 |
0.91 |
|
Petralia, Logan J (16)
|
NINAYE |
24.75 |
NS |
- |
|
Wells, Ben A (15)
|
NISKAN |
25.39 |
NS |
- |