| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Koehle, Evan J (9)
|
MANLAC |
46.83 |
40.67 |
-6.16 |
| 1 |
Rosenbach, Liam J (10)
|
MAHAY |
42.56 |
44.34 |
1.78 |
| 2 |
Barry, Patrick J (10)
|
MAMSA |
47.68 |
41.12 |
-6.56 |
| 2 |
Lehan, Colin S (10)
|
MAHAC |
45.30 |
45.00 |
-0.30 |
| 3 |
Garber, Timothy J (10)
|
MAEPAC |
|
42.28 |
- |
| 3 |
Shives, Connor B (10)
|
MAHAY |
44.15 |
46.94 |
2.79 |
| 4 |
Yan, Ivan N (10)
|
MAMSA |
46.87 |
42.79 |
-4.08 |
| 5 |
Buehler, Grant A (9)
|
MAKA |
|
43.41 |
- |
| 6 |
Soltanov, Yaroslav A (9)
|
MAHAC |
|
43.64 |
- |
| 7 |
Coleman, Ethan J (9)
|
MAPSC |
|
46.85 |
- |
| 8 |
Conway, Quinn D (10)
|
MAPSC |
|
48.76 |
- |
| 9 |
Deng, Jerry Y (9)
|
MAMSA |
|
48.85 |
- |
| 10 |
Zheng, Hadrian T (9)
|
MANLAC |
|
49.01 |
- |
| 11 |
Brace, Colton (10)
|
MAHAC |
|
1:05.05 |
- |
|
Yanchevskiy, Edvard E (9)
|
MAHAC |
|
DQ |
- |
|
Yu, Evan H (10)
|
MAMSA |
1:14.71 |
DQ |
- |