| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Zimmerman, Aaliyah D (12)
|
MAUN-0 |
31.24 |
30.45 |
-0.79 |
| 1 |
Araiza, Berenice L (12)
|
MACVAC |
32.46 |
32.95 |
0.49 |
| 1 |
Cramer, Aubrey G (11)
|
MACVAC |
35.83 |
35.70 |
-0.13 |
| 2 |
Good, Georgia L (12)
|
MDFAST |
31.82 |
32.10 |
0.28 |
| 2 |
Colina, Rondreizele (12)
|
MACVAC |
32.91 |
33.98 |
1.07 |
| 2 |
DeGirolamo, Katherine A (11)
|
MDFAST |
36.92 |
36.84 |
-0.08 |
| 3 |
Boone, Mary E (11)
|
MACVAC |
37.76 |
36.90 |
-0.86 |
| 4 |
Carliss, Sophia E (11)
|
MDFAST |
37.91 |
37.11 |
-0.80 |
| 5 |
Orcutt, Holly H (12)
|
MDFAST |
43.57 |
39.32 |
-4.25 |
| 6 |
Flores, Jasmin A (12)
|
MDFAST |
39.23 |
41.36 |
2.13 |
| 7 |
Hoffman, Ella G (12)
|
MDFAST |
|
42.22 |
- |
| 8 |
Welker, Harper K (11)
|
MDFAST |
|
42.95 |
- |
| 9 |
Goldman-Smith, Olivia C (11)
|
MAGETY |
50.30 |
45.41 |
-4.89 |
| 10 |
Wainberg, Brianna P (11)
|
MDFAST |
|
47.69 |
- |
| 11 |
Delcid, Kayla N (11)
|
MDFAST |
|
52.84 |
- |
|
Coombs, Madeline J (12)
|
MAKA |
|
DQ |
- |