| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Shaffer, Elizabeth L (14)
|
AMNHA |
2:52.17 |
2:43.24 |
-8.93 |
| 2 |
Akar, Eliana (14)
|
AMPEAQ |
2:58.43 |
2:50.74 |
-7.69 |
| 3 |
Kennedy, Delaney S (14)
|
AMANSC |
2:56.85 |
2:52.64 |
-4.21 |
| 4 |
Dickson, Eleah A (14)
|
AMPRA |
2:30.85 |
2:54.17 |
23.32 |
| 5 |
Anderson, Ariana A (13)
|
AMPRA |
2:59.43 |
2:55.37 |
-4.06 |
| 6 |
Milliron, Marissa J (14)
|
AMANSC |
3:03.05 |
2:57.30 |
-5.75 |
| 7 |
Hull, Marideth G (13)
|
AMMOON |
3:01.70 |
2:57.47 |
-4.23 |
| 8 |
O'Connor, Brooklyn E (13)
|
AMRXA |
2:50.90 |
2:57.63 |
6.73 |
| 9 |
Gilligan, Rena A (13)
|
AMMOON |
2:56.93 |
2:59.39 |
2.46 |
| 10 |
Kass, Savanna L (13)
|
AMRXA |
3:03.27 |
2:59.89 |
-3.38 |
| 11 |
Anderson, Braleigh A (14)
|
AMBPR |
3:08.45 |
3:04.59 |
-3.86 |
| 12 |
Pazuchanics, Kathryn E (13)
|
AMPTSC |
2:59.22 |
3:05.01 |
5.79 |
| 13 |
Laman, Sara V (13)
|
AMPEAQ |
3:12.94 |
3:05.14 |
-7.80 |
| 14 |
Pastor, Arienne P (14)
|
AMANSC |
3:06.21 |
3:05.25 |
-0.96 |
| 15 |
Coleman, Cassandra J (13)
|
AMPEAQ |
3:07.72 |
3:05.31 |
-2.41 |
| 16 |
Houser, McKenna L (13)
|
AMMOON |
3:06.57 |
3:07.83 |
1.26 |
| 17 |
Vales, Elizabeth M (13)
|
AMANSC |
3:16.37 |
3:13.10 |
-3.27 |
| 18 |
Medjimorec, Megan E (14)
|
AMPRA |
3:08.92 |
3:13.48 |
4.56 |