| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Webb, Margret C (12)
|
NCUN-0 |
29.64 |
29.13 |
-0.51 |
| 2 |
Riley, Erin N (12)
|
NCTAC |
30.39 |
29.56 |
-0.83 |
| 3 |
Cureton, Taylor L (11)
|
NCTAC |
31.02 |
29.71 |
-1.31 |
| 4 |
Schmid, Abigail L (15)
|
NCTAC |
31.85 |
30.86 |
-0.99 |
| 5 |
Long, Yzabella B (11)
|
NCTAC |
33.88 |
33.47 |
-0.41 |
| 6 |
Hamilton, Andria L (13)
|
NCASTF |
36.97 |
36.33 |
-0.64 |
| 7 |
Gilbert, Catherine L (14)
|
NCTAC |
40.36 |
37.64 |
-2.72 |
| 8 |
Ciancarelli, Sophia C (15)
|
NCTAC |
|
37.76 |
- |
| 9 |
Brown, Margaret H (11)
|
NCGFSC |
40.06 |
37.91 |
-2.15 |
| 10 |
Tuong, Katie D (11)
|
NCTAC |
46.45 |
41.55 |
-4.90 |
| 11 |
Kalika, Abigail R (11)
|
NCTAC |
44.43 |
42.66 |
-1.77 |
| 12 |
Arndts, Gray C (11)
|
NCTAC |
40.95 |
43.44 |
2.49 |
| 13 |
Valent, Hope V (11)
|
NCTAC |
42.91 |
46.37 |
3.46 |
|
Medsker, Emily (11)
|
NCTAC |
43.28 |
NS |
- |
|
Baric, Maria F (16)
|
NCUN-0 |
26.73 |
NS |
- |