| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Whitmire, Eliza P (14)
|
NCATOM |
4:43.55 |
4:34.34 |
-9.21 |
| 2 |
Krumanocker, Kelsey R (17)
|
NCCVAC |
4:45.43 |
4:43.04 |
-2.39 |
| 3 |
Boone, Savannah H (16)
|
NCATOM |
4:51.38 |
4:46.75 |
-4.63 |
| 4 |
Texer, Sadie P (17)
|
NCCVAC |
4:50.22 |
4:46.96 |
-3.26 |
| 5 |
Kudela, Amelia R (15)
|
NCUN-N |
5:08.15 |
5:04.60 |
-3.55 |
| 6 |
Watson, Dana R (15)
|
NCCVAC |
5:07.42 |
5:05.39 |
-2.03 |
| 7 |
Dunning, Catherine S (16)
|
NCBAD |
5:18.87 |
5:07.76 |
-11.11 |
| 8 |
Ingalls, Renny C (15)
|
NCATOM |
5:31.90 |
5:12.72 |
-19.18 |
| 9 |
Sellars, Sydney L (16)
|
NCQCD |
5:24.59 |
5:17.32 |
-7.27 |
| 10 |
Stewart, Myers E (18)
|
NCGG |
|
6:05.32 |
- |
| 11 |
Taylor, Emma G (12)
|
NCGG |
|
6:18.18 |
- |
| 12 |
Ellis, Egypt A (15)
|
NCQCD |
|
6:40.94 |
- |