| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Brown, Nya J (10)
|
SCSWIM |
3:19.62 |
3:12.18 |
-7.44 |
| 2 |
Luedke, Avery M (10)
|
NCTEAM |
3:23.02 |
3:14.45 |
-8.57 |
| 3 |
Crisp, Caroline P (10)
|
NCWA |
3:18.79 |
3:20.85 |
2.06 |
| 4 |
Deveaux, Kennedy A (10)
|
SCSWIM |
|
3:25.59 |
- |
| 5 |
Luker, Elizabeth D (10)
|
NCSAIL |
3:45.77 |
3:35.78 |
-9.99 |
| 6 |
Baumgartner, Campbell J (10)
|
NCMSA |
|
3:38.14 |
- |
| 7 |
Holland, Rylan A (10)
|
NCSAIL |
|
3:42.92 |
- |
| 8 |
Overturf, Addison N (9)
|
NCSAIL |
|
3:44.50 |
- |
| 9 |
Delea, Kathryn E (9)
|
NCUN-0 |
|
3:45.20 |
- |
| 10 |
Miller, Catherine F (10)
|
NCHPSC |
4:07.16 |
3:53.20 |
-13.96 |
| 11 |
Rojas, Anne M (8)
|
NCTEAM |
3:43.41 |
3:57.62 |
14.21 |
| 12 |
Lawrence, Amelia S (10)
|
NCWA |
3:55.73 |
4:00.87 |
5.14 |
| 13 |
Wasson, Hannah L (7)
|
NCCVAC |
|
4:02.86 |
- |
| 14 |
Hollar, Caylee A (8)
|
NCCVAC |
|
4:06.52 |
- |
| 15 |
Kaduson, Margo S (10)
|
NCMSA |
|
4:09.76 |
- |
| 16 |
Luedke, Tatum M (8)
|
NCTEAM |
4:06.26 |
4:11.19 |
4.93 |
| 17 |
Blamy, Lauren P (10)
|
NCMSA |
|
4:11.50 |
- |
| 18 |
Guenther, Katherine R (8)
|
NCATOM |
|
4:24.29 |
- |
| 19 |
Malarney, Madison C (8)
|
NCTEAM |
4:53.54 |
5:12.02 |
18.48 |
|
Ruelas Reyes, Samantha (10)
|
NCHPSC |
3:57.66 |
DQ |
- |
|
Pratt, Sloan I (10)
|
NCMSA |
|
NS |
- |