| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Sweet, Eli B (9)
|
NCGG |
38.55 |
39.26 |
0.71 |
| 2 |
Repella, Benjamin M (9)
|
NCATOM |
44.49 |
42.65 |
-1.84 |
| 3 |
Leftwich, Tucker G (10)
|
NCGG |
43.79 |
43.28 |
-0.51 |
| 4 |
Puri, Varun V (10)
|
NCATOM |
45.78 |
45.18 |
-0.60 |
| 5 |
Anderson, Cameron L (10)
|
NCQCD |
46.85 |
46.88 |
0.03 |
| 6 |
Seraz, Lucas I (10)
|
NCCVAC |
49.69 |
48.03 |
-1.66 |
| 7 |
MacGregor, Jackson D (10)
|
NCATOM |
47.66 |
49.74 |
2.08 |
| 8 |
Davis, Tyler C (10)
|
NCATOM |
47.81 |
50.21 |
2.40 |
| 9 |
Stinson, Alexander J (10)
|
NCATOM |
49.20 |
50.69 |
1.49 |
| 10 |
Boston, Emory E (10)
|
NCQCD |
49.06 |
51.81 |
2.75 |
| 11 |
Hill, Aidan J (9)
|
NCCVAC |
58.74 |
56.56 |
-2.18 |
| 12 |
Babani, Athan C (10)
|
NCATOM |
56.85 |
56.73 |
-0.12 |
| 13 |
Legnosky, Noah H (9)
|
NCGG |
1:04.48 |
57.74 |
-6.74 |
| 14 |
Richards, Clayton J (9)
|
NCATOM |
1:03.09 |
59.99 |
-3.10 |
| 15 |
Pace, Atticus M (9)
|
NCWST |
|
1:01.70 |
- |
|
MacGregor, Lucas W (8)
|
NCATOM |
|
DQ |
- |
|
VanDuzer, John M (10)
|
NCLENR |
1:10.05 |
DQ |
- |
|
Gates, Lucas A (9)
|
NCGG |
|
NS |
- |