| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Rojas, Mary Frances J (8)
|
NCBAD |
|
24.41 |
- |
| 2 |
Johnson, Valerie D (7)
|
NCCVAC |
26.30 |
25.47 |
-0.83 |
| 3 |
Ramsey, Chloe L (8)
|
NCWA |
|
26.44 |
- |
| 4 |
Hollingsworth, Makenzie L (8)
|
NCSAC |
26.97 |
26.56 |
-0.41 |
| 5 |
Stancil, Ellie G (8)
|
NCGG |
25.86 |
26.62 |
0.76 |
| 6 |
Millsaps, Katherine G (6)
|
NCCVAC |
34.75 |
27.81 |
-6.94 |
| 7 |
Bartlett, Veronica D (7)
|
NCCVAC |
29.26 |
29.35 |
0.09 |
| 8 |
Beltran-Trejo, Fernanda (7)
|
NCCVAC |
30.71 |
31.95 |
1.24 |
| 9 |
Young, Makayla O (8)
|
NCQCD |
31.56 |
33.22 |
1.66 |
| 10 |
McCraw, Reese M (6)
|
NCRCST |
38.19 |
34.76 |
-3.43 |
| 11 |
McPherson, Madeline R (7)
|
NCSAC |
|
39.96 |
- |
|
Millsaps, Caroline M (5)
|
NCCVAC |
|
DQ |
- |
|
Doerger, Eleanor A (7)
|
NCNMA |
|
DQ |
- |
|
Gladden, Averie D (8)
|
NCNMA |
|
DQ |
- |
|
Wilson, Camille (6)
|
NCNMA |
|
DQ |
- |
|
Stawecki, Riley A (8)
|
NCLENR |
36.00 |
DQ |
- |
|
Hernandez, Diana (6)
|
NCCVAC |
|
NS |
- |
|
Borders, Merrin J (7)
|
NCSAC |
|
NS |
- |
|
Sheets, Brooke (8)
|
NCAQTC |
|
NS |
- |