| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Pote, Jacob D (12)
|
NCSAIL |
36.02 |
34.26 |
-1.76 |
| 2 |
Akins, Liam K (12)
|
NCATOM |
35.51 |
35.30 |
-0.21 |
| 3 |
Bucci, Bryan C (12)
|
NCATOM |
38.15 |
37.40 |
-0.75 |
| 4 |
Smith, Gunner L (11)
|
NCCVAC |
41.42 |
38.64 |
-2.78 |
| 5 |
Sellars, Elijah A (11)
|
NCQCD |
41.22 |
38.84 |
-2.38 |
| 6 |
Harris, Hudson C (11)
|
NCTEAM |
40.27 |
39.07 |
-1.20 |
| 7 |
Williams, Benjamin B (12)
|
NCSAIL |
38.96 |
39.26 |
0.30 |
| 8 |
Herger, Wyatt K (12)
|
NCATOM |
40.43 |
39.59 |
-0.84 |
| 9 |
Woolwine, Nathan A (12)
|
NCSAIL |
47.71 |
41.11 |
-6.60 |
| 10 |
Hedrick, Noah A (11)
|
NCCVAC |
43.23 |
41.80 |
-1.43 |
| 11 |
Anderson, Austin B (12)
|
NCSAIL |
42.56 |
42.36 |
-0.20 |
| 12 |
Enohmbi, Emmanuel T (12)
|
NCMSA |
43.69 |
42.78 |
-0.91 |
| 13 |
Lin, Richard (12)
|
NCMSA |
|
43.09 |
- |
| 14 |
Cassidy, Peter B (11)
|
NCATOM |
44.06 |
43.83 |
-0.23 |
| 15 |
Hall, James C (11)
|
NCATOM |
43.42 |
43.84 |
0.42 |
| 16 |
Bartlett, Jack W (12)
|
NCCVAC |
46.00 |
43.87 |
-2.13 |
| 17 |
Chopra, Viraj V (11)
|
NCMSA |
42.88 |
44.12 |
1.24 |
| 18 |
Delaney, Matthew J (11)
|
NCUN-N |
|
46.47 |
- |
| 19 |
Zon, Alexander L (12)
|
NCTEAM |
52.96 |
48.11 |
-4.85 |
| 20 |
Rhodes, Hobson C (11)
|
NCTEAM |
50.99 |
49.63 |
-1.36 |
| 21 |
Williams, Elijah (12)
|
NCWA |
48.99 |
49.64 |
0.65 |
| 22 |
Lee, Harrison J (11)
|
NCWA |
|
50.25 |
- |
| 23 |
Jones, Lawrence E (11)
|
SCGVST |
|
50.29 |
- |
| 24 |
Hughes, Henry A (11)
|
NCMSA |
1:01.12 |
55.30 |
-5.82 |
| 25 |
Ramirez, Daniel A (12)
|
NCCVAC |
1:08.70 |
1:01.55 |
-7.15 |
|
Martin, Benjamin T (12)
|
NCMSA |
36.54 |
DQ |
- |
|
Knesel, Eric A (12)
|
NCMSA |
1:05.01 |
NS |
- |
|
Thadakamadla, Sushrut (11)
|
NCUN-N |
|
NS |
- |