| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Donlan, Molly J (12)
|
NC1977 |
54.51 |
54.88 |
0.37 |
| 2 |
Druhan, Abigail G (12)
|
NC1990 |
57.98 |
58.67 |
0.69 |
| 3 |
Martinson, Kara N (12)
|
NC1994 |
1:02.74 |
59.73 |
-3.01 |
| 4 |
Sleime, Leyla C (12)
|
NC1990 |
1:00.38 |
1:00.25 |
-0.13 |
| 5 |
Floyd, Charley C (12)
|
NC1977 |
1:03.28 |
1:00.65 |
-2.63 |
| 6 |
VanNote, Carly I (11)
|
NC1977 |
1:00.43 |
1:00.71 |
0.28 |
| 7 |
Gehrig, Julia J (12)
|
NC1994 |
1:03.31 |
1:00.92 |
-2.39 |
| 8 |
DeCann, Brooklyn G (12)
|
NC1977 |
1:03.14 |
1:02.24 |
-0.90 |
| 9 |
Hollmeyer, Abigail L (12)
|
NC1990 |
1:04.73 |
1:02.27 |
-2.46 |
| 10 |
Lindauer, Sophia L (12)
|
NC1990 |
1:05.05 |
1:02.52 |
-2.53 |
| 11 |
Ashi, Sophia (11)
|
NC1990 |
1:02.93 |
1:02.60 |
-0.33 |
| 12 |
Fan, Victoria (11)
|
NC1994 |
1:02.76 |
1:02.73 |
-0.03 |
| 13 |
Dalton, Mary B (12)
|
NC1990 |
1:01.96 |
1:02.74 |
0.78 |
| 14 |
Bernardo, Lauren J (12)
|
NC1977 |
1:06.65 |
1:02.95 |
-3.70 |
| 15 |
Hollar, Lauren B (12)
|
NC1977 |
1:05.52 |
1:03.73 |
-1.79 |
| 16 |
McFee, Lizzie (12)
|
NC1990 |
1:05.41 |
1:03.84 |
-1.57 |
| 17 |
Chin, Ella S (12)
|
NC1977 |
1:03.00 |
1:03.95 |
0.95 |
| 18 |
Bombien, Ansley G (12)
|
NC1977 |
1:03.03 |
1:04.10 |
1.07 |
| 19 |
Christensen, Ryleigh E (12)
|
NC1977 |
1:06.32 |
1:04.18 |
-2.14 |
| 20 |
LeGrand, Francesca M (11)
|
NC1994 |
1:05.75 |
1:04.31 |
-1.44 |
| 21 |
Monteith, Macarthy M (11)
|
NC1977 |
1:05.69 |
1:04.51 |
-1.18 |
| 22 |
Williams, Sarah R (12)
|
NC1990 |
1:04.83 |
1:05.10 |
0.27 |
| 23 |
Thome, Paige K (11)
|
NC1994 |
1:06.49 |
1:05.34 |
-1.15 |
| 24 |
Bujoreanu, Sophia E (11)
|
NC1990 |
1:08.71 |
1:06.04 |
-2.67 |
| 25 |
Holden, Lanie C (11)
|
NC1990 |
1:05.47 |
1:07.10 |
1.63 |
|
Topp, Makenna G (12)
|
NC1990 |
56.76 |
NS |
- |
|
Sandstrom, Madison A (11)
|
NC1994 |
1:01.16 |
NS |
- |