| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Texer, Sadie P (17)
|
NCCVAC |
2:35.56 |
2:32.52 |
-3.04 |
| 2 |
Turley, Olivia G (16)
|
NCTEAM |
2:33.29 |
2:35.85 |
2.56 |
| 3 |
Marquardt, Eleanore K (16)
|
NCATOM |
2:54.49 |
2:36.47 |
-18.02 |
| 4 |
Marshall, Christina L (18)
|
NCEAC |
2:47.61 |
2:39.13 |
-8.48 |
| 5 |
Stolberg, Emma E (12)
|
NCUN-0 |
|
2:39.32 |
- |
| 6 |
Vendal, Ella K (15)
|
NCATOM |
2:47.61 |
2:43.84 |
-3.77 |
| 7 |
Daniels, Grace O (12)
|
NCATOM |
|
2:55.11 |
- |
| 8 |
Watson, Darah E (17)
|
NCCVAC |
2:55.42 |
2:56.82 |
1.40 |
| 9 |
Tipton, Tamra N (12)
|
NCMSA |
|
2:57.97 |
- |
| 10 |
Middlesworth, Isabelle M (13)
|
NCATOM |
3:01.30 |
2:58.64 |
-2.66 |
| 11 |
Powell, Katelyn A (15)
|
NCATOM |
|
3:17.36 |
- |
|
Mayhall, Kylie R (15)
|
NCEAC |
|
NS |
- |