| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Eckert, Charlotte N (12)
|
NCMAC |
37.04 |
33.96 |
-3.08 |
| 2 |
LeGrand, Francesca M (12)
|
NCMAC |
36.05 |
35.66 |
-0.39 |
| 3 |
Briggs, Reese E (12)
|
NCMAC |
37.14 |
35.69 |
-1.45 |
| 4 |
Brewer, Ashtyn B (11)
|
NCMAC |
36.30 |
35.75 |
-0.55 |
| 5 |
Neal, Kayman R (12)
|
NCMAC |
38.09 |
35.80 |
-2.29 |
| 6 |
Taylor, Ainsley G (12)
|
PVNCAP |
35.52 |
36.36 |
0.84 |
| 7 |
Cardwell, Ava E (11)
|
NCMAC |
37.66 |
36.38 |
-1.28 |
| 8 |
Klamfoth, Avery R (11)
|
NCMAC |
37.80 |
36.51 |
-1.29 |
| 9 |
Sarrantonio, Ali (12)
|
NCMAC |
37.03 |
36.93 |
-0.10 |
| 10 |
Monteith, Macarthy M (12)
|
NCMAC |
37.22 |
37.18 |
-0.04 |
| 11 |
Martin, Nora C (12)
|
NCMAC |
36.55 |
37.46 |
0.91 |
| 12 |
Kennedy, Laura K (12)
|
NCTYDE |
38.74 |
37.63 |
-1.11 |
| 13 |
McCarter, Macie H (11)
|
NCMAC |
37.56 |
38.08 |
0.52 |
| 14 |
Serang, Lulu A (11)
|
NCTYDE |
40.23 |
38.44 |
-1.79 |
| 15 |
Brown, Keegan H (12)
|
NCTYDE |
37.03 |
38.70 |
1.67 |
| 16 |
Moehrke, Brooke A (11)
|
NCTYDE |
39.76 |
41.92 |
2.16 |