| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Jellig, Conor M (17)
|
MAUN-M |
53.91 |
53.98 |
0.07 |
| 2 |
King, David W (15)
|
VAGATR |
57.39 |
55.70 |
-1.69 |
| 3 |
Gabriele, Noah T (15)
|
VAGATR |
57.62 |
56.45 |
-1.17 |
| 4 |
Kidd, Tanner C (13)
|
VAGATR |
58.04 |
56.90 |
-1.14 |
| 5 |
Wang, Richard H (13)
|
VAGATR |
59.79 |
57.93 |
-1.86 |
| 6 |
Alexander, Jonathan D (15)
|
VAGATR |
57.23 |
58.02 |
0.79 |
| 7 |
Warnagiris, Joseph J (13)
|
VAWST |
58.69 |
58.13 |
-0.56 |
| 8 |
Fowler, Henry H (17)
|
VAWST |
1:00.62 |
58.98 |
-1.64 |
| 9 |
King, Dylan J (15)
|
VAGATR |
1:02.33 |
1:01.45 |
-0.88 |
| 10 |
King, Brayden D (13)
|
VAGATR |
1:05.96 |
1:03.80 |
-2.16 |
| 11 |
Bledsoe, Samuel C (13)
|
VAGATR |
1:06.78 |
1:04.49 |
-2.29 |
| 12 |
Oakey, Phillip S (17)
|
VAGATR |
1:04.52 |
1:04.99 |
0.47 |
| 13 |
Strickler, Sawyer J (14)
|
VAGATR |
1:04.61 |
1:05.22 |
0.61 |
| 14 |
Tharp, Trevor S (14)
|
VAGATR |
1:17.19 |
1:06.51 |
-10.68 |
| 15 |
Martin, Cole S (15)
|
VAGATR |
1:05.57 |
1:06.72 |
1.15 |
| 16 |
Grimes, Colby T (14)
|
VAGATR |
1:10.88 |
1:07.39 |
-3.49 |
|
Wenger, Isaac S (15)
|
VAGATR |
1:16.35 |
DQ |
- |