| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Noecker, Elle A (11)
|
NITWST |
40.26 |
38.63 |
-1.63 |
| 2 |
Sarginson, Piper (12)
|
BROC |
44.78 |
46.70 |
1.92 |
| 3 |
Fritsch, Natalie A (12)
|
NIUN-T |
46.50 |
46.99 |
0.49 |
| 4 |
Yarnell, Natalie (12)
|
BROC |
|
47.39 |
- |
| 5 |
Moldenhauer, Paige A (11)
|
NIEAST |
55.01 |
49.68 |
-5.33 |
| 6 |
Sainsbury, Meghan A (12)
|
NITWST |
1:00.87 |
51.16 |
-9.71 |
| 7 |
Mitchell, Gwenyth E (11)
|
NIEMAC |
49.55 |
51.43 |
1.88 |
| 8 |
Van Dyk, Anna (11)
|
BROC |
51.31 |
53.52 |
2.21 |
| 9 |
Del Regno, Jaina R (12)
|
NIRAYS |
1:00.01 |
53.56 |
-6.45 |
| 10 |
Hauser-Howells, Lillian K (12)
|
NITTSC |
53.17 |
54.77 |
1.60 |
| 11 |
Baker, Sofia L (11)
|
NITWST |
|
54.78 |
- |
| 12 |
Mruzik, Lillian G (11)
|
NIEAST |
|
56.25 |
- |
| 13 |
Black, Alexandra (11)
|
BROC |
46.00 |
56.28 |
10.28 |
| 14 |
Sweeney, Faye (11)
|
NIRAYS |
|
1:03.26 |
- |
|
Berdysiak, Elizabeth C (12)
|
NICLSC |
44.95 |
DQ |
- |
|
Ehrmann, Jenna R (12)
|
NICLSC |
50.14 |
NS |
- |
|
Griffin, Haley A (11)
|
NITTSC |
53.20 |
NS |
- |
|
Leslie, Julia G (12)
|
NITTSC |
55.41 |
NS |
- |