| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Mombrea, Joseph R (16)
|
NIWWSC |
50.00 |
49.64 |
-0.36 |
| 2 |
Mixon, Matthew D (18)
|
NIWWSC |
52.69 |
51.45 |
-1.24 |
| 3 |
Miller, Dylan M (17)
|
NIWWSC |
51.73 |
52.27 |
0.54 |
| 4 |
Maciejewski, Jacob R (16)
|
NIRAYS |
54.95 |
55.30 |
0.35 |
| 5 |
Clouser, Kameron D (17)
|
NIBCSR |
55.12 |
55.51 |
0.39 |
| 6 |
Crego, Alex N (15)
|
NICLSC |
1:00.00 |
1:02.24 |
2.24 |
| 7 |
Wiegand, Jakob W (15)
|
NITTSC |
1:01.46 |
1:02.48 |
1.02 |
| 8 |
Nienhaus, Jack (15)
|
NISHAQ |
1:05.54 |
1:02.94 |
-2.60 |
| 9 |
Madge, Brent A (15)
|
NIRAYS |
1:15.53 |
1:13.41 |
-2.12 |
|
Switalski, Nicholas M (15)
|
NITTSC |
1:03.86 |
NS |
- |