| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Tedeschi, Roman (11)
|
NIAMTS |
41.39 |
36.50 |
-4.89 |
| 2 |
Carpenter, Trenten E (12)
|
NIRAYS |
40.20 |
39.79 |
-0.41 |
| 3 |
Szmanda, Cristopher D (11)
|
NIRAYS |
41.85 |
42.64 |
0.79 |
| 4 |
Woodford, Derek (11)
|
NISHAQ |
50.07 |
44.14 |
-5.93 |
| 5 |
LaMoy, Hunter P (12)
|
NIRAYS |
43.90 |
44.52 |
0.62 |
| 6 |
Natiella, Noah (12)
|
NIAMTS |
42.97 |
44.57 |
1.60 |
| 7 |
Avino, Evan A (12)
|
NISTAR |
1:02.86 |
58.09 |
-4.77 |
| 8 |
Gross, Noah R (11)
|
NISTAR |
45.73 |
58.13 |
12.40 |
| 9 |
Spaeth, Alexander J (12)
|
NISTAR |
1:00.01 |
58.45 |
-1.56 |
| 10 |
King, Austin R (11)
|
NIWWSC |
|
58.76 |
- |
|
Coen, Liam C (11)
|
NISTAR |
39.92 |
DQ |
- |
|
Roche, Robert (11)
|
NISTAR |
47.38 |
NS |
- |