| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Moran, Aiden T (8)
|
NIUN-C |
33.07 |
32.77 |
-0.30 |
| 2 |
Young-Mandiak, Atticus F (9)
|
NISTAR |
33.10 |
33.36 |
0.26 |
| 3 |
Garcia, Brady E (9)
|
NITTSC |
34.76 |
36.17 |
1.41 |
| 4 |
Coen, Liam C (10)
|
NISTAR |
36.29 |
36.84 |
0.55 |
| 5 |
Coen, Jack D (10)
|
NISTAR |
37.65 |
38.16 |
0.51 |
| 6 |
Nienhaus, Matthew (8)
|
NISHAQ |
41.38 |
40.19 |
-1.19 |
| 7 |
McCann, Timothy A (9)
|
NISTAR |
41.33 |
41.03 |
-0.30 |
| 8 |
Parsnick, William Z (7)
|
NICLSC |
47.12 |
41.54 |
-5.58 |
| 8 |
Szmanda, Cristopher D (10)
|
NIRAYS |
40.09 |
41.54 |
1.45 |
| 10 |
Lorenz, Charles D (9)
|
NITTSC |
41.29 |
41.57 |
0.28 |
| 11 |
Reygers, Camden M (8)
|
NIWWSC |
42.97 |
42.11 |
-0.86 |
| 12 |
Herington, Mason A (10)
|
NITTSC |
45.00 |
42.54 |
-2.46 |
| 13 |
King, Emmett M (8)
|
NITTSC |
46.52 |
42.61 |
-3.91 |
| 14 |
Madden, Tristen S (9)
|
NIFALC |
|
46.59 |
- |
| 15 |
Goldstein, Cole M (9)
|
NISTAR |
47.77 |
46.81 |
-0.96 |
| 16 |
Roche, Robert (10)
|
NISTAR |
50.27 |
47.70 |
-2.57 |
| 17 |
Ruffolo, Trenton J (8)
|
NIWWSC |
48.88 |
51.70 |
2.82 |
| 18 |
Pyc, Jaxen R (7)
|
NIWWSC |
|
54.33 |
- |
| 19 |
Charlier, Andre D (7)
|
NITTSC |
53.98 |
54.37 |
0.39 |
| 20 |
Young, Thaddeus M (7)
|
NISTAR |
56.66 |
58.55 |
1.89 |
| 21 |
King, Isaac (6)
|
NITTSC |
59.15 |
1:04.54 |
5.39 |
| 22 |
Gray, Richard L (10)
|
NISTAR |
1:00.10 |
1:06.19 |
6.09 |
| 23 |
Quiambao, Marc (10)
|
NITTSC |
|
1:09.15 |
- |
| 24 |
Stillman, Jack (8)
|
NITTSC |
|
1:29.73 |
- |
|
Tebeau, Jack M (9)
|
NISTAR |
38.62 |
NS |
- |