| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Rutherford, Eli J (12)
|
VACA-Y |
28.07 |
27.54 |
-0.53 |
| 2 |
Wolk, Max A (12)
|
NCNCAC |
27.85 |
27.58 |
-0.27 |
| 3 |
Arcarese, James R (12)
|
VANOVA |
28.96 |
28.00 |
-0.96 |
| 4 |
Charlton, Benjamin S (12)
|
VACA-Y |
28.84 |
28.58 |
-0.26 |
| 5 |
Van Deren, Jackson R (12)
|
VANOVA |
28.50 |
28.82 |
0.32 |
| 6 |
Wong, Preston S (12)
|
VANOVA |
30.73 |
28.91 |
-1.82 |
| 7 |
Su, Jacob A (12)
|
NCNCAC |
29.80 |
29.12 |
-0.68 |
| 8 |
Morgan, Jackson W (12)
|
VANOVA |
30.08 |
29.25 |
-0.83 |
| 9 |
Lowry, Chance P (12)
|
VANOVA |
29.76 |
29.32 |
-0.44 |
| 10 |
Mitler, Charles G (12)
|
NCNCAC |
30.25 |
29.70 |
-0.55 |
| 11 |
Hilton, Charles P (12)
|
VANOVA |
30.95 |
29.90 |
-1.05 |
| 12 |
Sardesai, Nolan A (12)
|
VANOVA |
30.20 |
30.33 |
0.13 |
| 13 |
Courtois, Shane S (12)
|
MDFSC |
30.66 |
30.39 |
-0.27 |
| 14 |
Xie, William (12)
|
VACA-Y |
30.30 |
30.40 |
0.10 |
| 15 |
Harrington, Luke P (12)
|
VANOVA |
31.10 |
30.63 |
-0.47 |
| 16 |
Jones, Jonathan F (12)
|
NCNCAC |
31.02 |
30.94 |
-0.08 |
| 17 |
Ademola, Oluwaseun J (12)
|
MDFSC |
31.50 |
31.11 |
-0.39 |
| 18 |
Binter, Zachary W (12)
|
VACA-Y |
30.56 |
31.39 |
0.83 |
| 19 |
Du, Andrew Z (12)
|
VAHOKI |
33.60 |
31.88 |
-1.72 |
| 20 |
Ackerly, John P (12)
|
VANOVA |
29.99 |
31.90 |
1.91 |
| 21 |
Dove, Dakota M (12)
|
MDFSC |
33.50 |
32.35 |
-1.15 |
| 22 |
Holt, Dylan T (12)
|
MDFSC |
31.64 |
33.28 |
1.64 |
| 23 |
Porter, Owen H (12)
|
VALY |
35.08 |
34.09 |
-0.99 |
| 24 |
Wu, Caleb C (12)
|
VALY |
35.03 |
34.28 |
-0.75 |
| 25 |
Cullaty, Gabriel R (12)
|
VACA-Y |
37.65 |
37.50 |
-0.15 |
| 26 |
Park, Moses (12)
|
VAHOKI |
39.89 |
38.69 |
-1.20 |
|
Miklos, Owen T (12)
|
VANOVA |
27.40 |
DQ |
- |
|
Wetzel, Damien (12)
|
VAVABR |
46.05 |
NS |
- |
|
Rogers, Mitchell T (12)
|
VALY |
29.93 |
NS |
- |
|
Hodges, Mason C (12)
|
VAHOKI |
39.16 |
NS |
- |