| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Schooling, Joseph I (25)
|
VAUN |
|
46.01 |
- |
| 2 |
Ivanov, Antani A (19)
|
VAVT |
|
46.69 |
- |
| 3 |
Ramadan, Youssef T (18)
|
VAVT |
|
46.72 |
- |
| 4 |
Manoff, Philip B (17)
|
VAVT |
|
46.96 |
- |
| 5 |
Bureau, Lucas A (26)
|
VAUN |
|
48.25 |
- |
| 6 |
Claesson, Henry A (18)
|
VAVT |
|
48.67 |
- |
| 7 |
Eichberg, Dylan A (18)
|
VAVT |
|
49.78 |
- |
| 8 |
Gutierrez, Gustavo G (21)
|
VAPRVT |
|
50.44 |
- |
| 9 |
Aguilar-Fernandez, Rodolfo J (21)
|
VAPRVT |
|
50.64 |
- |
| 10 |
Fonder, Khalil D (21)
|
VAVT |
|
51.17 |
- |
| 11 |
Lebed, Alex M (24)
|
VAUN |
|
51.21 |
- |
| 12 |
Panchak, Jonathan C (24)
|
VAPRVT |
|
51.24 |
- |
| 13 |
Neff, Alexander K (18)
|
VAVT |
|
52.46 |
- |
| 14 |
Myburgh, Collin A (18)
|
VAVT |
|
53.02 |
- |
| 15 |
Clark, Jaedin D (18)
|
VAVT |
|
53.56 |
- |
| 16 |
Englehart, William D (19)
|
VAUN |
|
56.90 |
- |