| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Lugar, George C (8)
|
VAGATR |
40.23 |
39.03 |
-1.20 |
| 2 |
Strupe, Fredric J (8)
|
NCTEAM |
50.67 |
44.34 |
-6.33 |
| 3 |
Hennaux, Jean-Pierre A (8)
|
SEKP |
47.54 |
46.73 |
-0.81 |
| 4 |
Hoyos, Benjamin T (8)
|
VASCAT |
58.70 |
54.22 |
-4.48 |
| 5 |
Du, Andrew Z (8)
|
VAHOKI |
|
55.97 |
- |
| 6 |
Tolin, Cooper C (8)
|
NCRSA |
|
57.49 |
- |
| 7 |
Brumback, Noah G (8)
|
NCRSA |
52.17 |
1:02.40 |
10.23 |
| 8 |
Nunn, Bo B (7)
|
NCEAC |
1:00.00 |
1:03.45 |
3.45 |
| 9 |
Feathers, Jacob M (8)
|
SEKP |
|
1:07.65 |
- |
| 10 |
Durbin, Finnegan P (8)
|
VAHOKI |
|
1:13.88 |
- |
|
Giovannini, Niccolo J (8)
|
VAUN-G |
|
NS |
- |