| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Zolnak, Scarlett E (10)
|
AMFAST |
37.19 |
38.91 |
1.72 |
| 2 |
Brumbaugh, Lillian L (10)
|
AMWAAC |
42.39 |
40.36 |
-2.03 |
| 3 |
Zakrzewski, Brinley C (10)
|
AMWAAC |
38.90 |
40.48 |
1.58 |
| 4 |
Kaminski, Elle K (10)
|
AMMMSA |
44.27 |
43.33 |
-0.94 |
| 5 |
Tom, Anika M (10)
|
AMFAST |
43.93 |
43.96 |
0.03 |
| 6 |
Peace, Meela J (10)
|
AMCSC |
49.32 |
44.64 |
-4.68 |
| 7 |
Tropeck, Allison B (9)
|
AMWAAC |
49.90 |
45.81 |
-4.09 |
| 8 |
Melocchi, Ava P (10)
|
AMSASC |
48.71 |
48.40 |
-0.31 |
| 9 |
Hoskins, Alaina G (9)
|
AMWHAT |
54.02 |
49.34 |
-4.68 |
| 10 |
Sullivan, Keelin A (10)
|
AMNAC |
48.95 |
51.66 |
2.71 |
| 11 |
Boone, Abby (10)
|
AMWHAT |
46.85 |
51.81 |
4.96 |
| 12 |
Hunsinger, Reese E (9)
|
AMFAST |
58.18 |
52.42 |
-5.76 |
|
Klutch, Alexis G (9)
|
AMBPR |
1:00.70 |
NS |
- |
|
Mattila, Grace I (9)
|
AMBPR |
53.23 |
NS |
- |
|
Kloc, Aleah (9)
|
AMCSC |
47.34 |
NS |
- |
|
Mascari, Lilliana R (9)
|
AMSASC |
50.86 |
NS |
- |