| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Uhlman, Alyssa A (11)
|
OHBASH |
28.73 |
28.23 |
-0.50 |
| 2 |
Sellinger, Lucille A (12)
|
OHBASH |
29.78 |
29.43 |
-0.35 |
| 3 |
Koetter, Sarah L (12)
|
OHBASH |
29.51 |
30.63 |
1.12 |
| 4 |
Detweiler, Yvette A (12)
|
OHBASH |
31.82 |
30.95 |
-0.87 |
| 5 |
Wallbrown, Mary J (12)
|
OHBASH |
32.19 |
30.97 |
-1.22 |
| 5 |
Yu, Grace (11)
|
OHBASH |
31.50 |
30.97 |
-0.53 |
| 7 |
Jain, Anushka K (12)
|
OHPCY |
31.29 |
31.18 |
-0.11 |
| 8 |
Mundhe, Kimaya * (12)
|
OHBASH |
31.85 |
31.56 |
-0.29 |
| 9 |
Gray, Janiyah B (12)
|
OHPCY |
33.84 |
32.67 |
-1.17 |
| 10 |
Froehle, Samantha R (12)
|
OHPCY |
35.05 |
32.85 |
-2.20 |
| 11 |
Meister, Emily K (12)
|
OHPCY |
36.28 |
33.16 |
-3.12 |
| 12 |
Lind, Tabitha R (12)
|
OHPCY |
|
34.30 |
- |
| 13 |
McCauley, Gabriella M (11)
|
OHPCY |
33.69 |
34.58 |
0.89 |
| 14 |
Buchanan, Aubrey M (11)
|
OHPCY |
37.73 |
34.71 |
-3.02 |
| 15 |
Hubbell, Keally P (12)
|
OHBASH |
34.85 |
35.99 |
1.14 |
| 16 |
Ihnen, Lucy K (11)
|
OHPCY |
|
36.78 |
- |
| 18 |
Wright, Mia L (11)
|
OHBASH |
39.07 |
39.12 |
0.05 |
| 19 |
Larson, Lily J (12)
|
OHPCY |
|
43.80 |
- |
| 20 |
Chrapliwy, Olivia J (11)
|
OHPCY |
|
47.39 |
- |