| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Spencer, Sarah O (13)
|
OHPCY |
2:43.76 |
2:43.21 |
-0.55 |
| 2 |
Schneider, Victoria C (13)
|
OHLAKY |
2:50.98 |
2:49.27 |
-1.71 |
| 3 |
Jutze, Molly E (14)
|
OHBASH |
3:10.76 |
3:07.98 |
-2.78 |
| 4 |
Ryan, Kate (13)
|
OHBASH |
|
3:11.94 |
- |
| 5 |
Sheanshang, Keegan C (13)
|
OHBASH |
3:14.57 |
3:12.69 |
-1.88 |
| 6 |
Kirby, MacKenzie I (13)
|
OHBASH |
3:21.51 |
3:17.63 |
-3.88 |
| 7 |
Centers, Alexa K (14)
|
OHCY |
3:19.10 |
3:19.21 |
0.11 |
| 8 |
Mount, Lauren L (13)
|
OHBASH |
3:09.37 |
3:23.22 |
13.85 |
| 9 |
Vander Veen, Kelly J (14)
|
OHPCY |
3:45.26 |
3:36.41 |
-8.85 |
|
Remsen, Aris S (13)
|
OHABLY |
3:22.33 |
NS |
- |
|
Gamble, Hazelle D (13)
|
OHPCY |
|
NS |
- |
|
Gabbert, Laney M (13)
|
OHSPY |
|
NS |
- |