| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Roy, Oliver J (17)
|
ADADDI |
25.44 |
24.13 |
-1.31 |
| 2 |
Schiavi, Gavin J (16)
|
ADNHA |
24.92 |
24.57 |
-0.35 |
| 3 |
Gordon-Stacey, Druhan M (19)
|
ADALBS |
25.32 |
25.10 |
-0.22 |
| 4 |
Silaika, Xavier R (15)
|
ADCPP |
25.98 |
25.48 |
-0.50 |
| 5 |
Scott, Eric D (17)
|
ADCPP |
26.68 |
25.49 |
-1.19 |
| 6 |
Travis, Matthew J (16)
|
ADCANA |
26.39 |
25.62 |
-0.77 |
| 7 |
LaFountain, Benjamin P (16)
|
ADALBS |
25.48 |
25.82 |
0.34 |
| 8 |
Boehmer, Ethan M (16)
|
ADCPP |
26.54 |
25.87 |
-0.67 |
| 9 |
Massa, Nicholas J (18)
|
ADNHA |
26.84 |
26.09 |
-0.75 |
| 10 |
Cruz-Figueroa, Eduardo (17)
|
ADCPP |
26.60 |
26.83 |
0.23 |
| 11 |
Catanzaro, Michael M (18)
|
ADCPP |
27.19 |
27.20 |
0.01 |
| 12 |
Mace, Christopher G (15)
|
ADCPP |
28.46 |
27.36 |
-1.10 |
| 13 |
Thompson, Dylan E (18)
|
ADSVP |
28.06 |
27.47 |
-0.59 |
| 14 |
Marcotullio, Patrick R (18)
|
ADADDI |
27.62 |
27.49 |
-0.13 |
| 15 |
Cazer, John A (15)
|
ADALBS |
27.04 |
27.92 |
0.88 |
| 16 |
Wong, Michael (15)
|
ADALBS |
27.45 |
28.36 |
0.91 |
| 17 |
Hyman, Nolan B (16)
|
ADNHA |
30.26 |
29.88 |
-0.38 |
| 18 |
Gioppo, Zachary A (15)
|
ADNHA |
32.09 |
31.81 |
-0.28 |
|
Kuo, Bryce P (15)
|
ADCPP |
26.93 |
NS |
- |
|
Jordan, Timothy A (16)
|
ADCPP |
26.59 |
NS |
- |
|
Herbinger, Devin R (17)
|
ADCPP |
25.63 |
NS |
- |