| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Connelly, Reagan E (12)
|
AMPRA |
5:59.60 |
5:45.35 |
-14.25 |
| 2 |
Ding, Reena R (12)
|
OHDCST |
6:01.05 |
5:55.45 |
-5.60 |
| 3 |
Bruner, Brynn A (12)
|
LEUN-L |
5:58.12 |
6:03.34 |
5.22 |
| 4 |
Schomaker, Anna J (12)
|
AMPRA |
6:07.47 |
6:12.12 |
4.65 |
| 5 |
Aung, Suri L (12)
|
OHOSSC |
6:15.19 |
6:14.19 |
-1.00 |
| 6 |
Shafer, Riley A (11)
|
AMPRA |
6:19.03 |
6:15.88 |
-3.15 |
| 7 |
Slauson, Alexandra M (11)
|
OHUN |
6:12.52 |
6:16.26 |
3.74 |
| 8 |
Harper, Ava E (12)
|
OHNAAC |
6:40.03 |
6:21.63 |
-18.40 |
| 9 |
Richards, Skylar M (11)
|
OHOSSC |
6:20.19 |
6:24.26 |
4.07 |
| 10 |
Pietropaolo, Sophia G (12)
|
AMPRA |
6:29.44 |
6:25.52 |
-3.92 |