| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Acuff, Sienna B (12)
|
NTDM |
32.48 |
30.62 |
-1.86 |
| 2 |
Zhang, Kayla J (10)
|
NTDM |
42.76 |
36.98 |
-5.78 |
| 3 |
La Fontaine, Diana M (11)
|
NTDM |
41.14 |
39.30 |
-1.84 |
| 4 |
Dehmer, Harper A (11)
|
NTDM |
42.56 |
40.20 |
-2.36 |
| 5 |
Celeste, Laura C (10)
|
NTDM |
41.98 |
41.84 |
-0.14 |
| 6 |
Morris, Claire M (10)
|
NTDM |
43.69 |
43.67 |
-0.02 |
| 7 |
Rodriguez, Emma E (11)
|
NTDM |
45.80 |
45.14 |
-0.66 |
| 8 |
Acuff, Isabella R (10)
|
NTDM |
57.15 |
47.28 |
-9.87 |
| 9 |
Knight, Pearce S (11)
|
NTDM |
|
52.29 |
- |
| 10 |
Christian, Elliot J (9)
|
NTDM |
55.04 |
58.05 |
3.01 |
| 11 |
Yip, Alyssa V (11)
|
NTDM |
|
58.82 |
- |
| 12 |
Fisher, Harmony M (10)
|
NTDM |
56.81 |
59.64 |
2.83 |
| 13 |
Bourland, Caroline F (9)
|
NTDM |
|
1:04.95 |
- |
|
Bourland, Carmella L (11)
|
NTDM |
|
DQ |
- |
|
Shirk, Amelia F (10)
|
NTDM |
|
DQ |
- |