| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
HAND, CASSIDY R (10)
|
NIGVST |
47.93 |
47.44 |
-0.49 |
| 2 |
MOLDENHAUER, PAIGE A (10)
|
NIEAST |
46.97 |
48.19 |
1.22 |
| 3 |
FLICKINGER, MARIA J (10)
|
NIEAST |
50.60 |
48.31 |
-2.29 |
| 4 |
SAND, MAKAYLA E (10)
|
NITWST |
53.33 |
50.13 |
-3.20 |
| 5 |
KERINS, BAILEY R (10)
|
NIHAMB |
54.22 |
52.70 |
-1.52 |
| 6 |
SPERRY, LOLA E (10)
|
NITTSC |
55.21 |
53.89 |
-1.32 |
| 7 |
DELCAMP, ASHLYNN R (10)
|
NIHHSC |
|
54.01 |
- |
| 8 |
MCGILL, ROSE M (10)
|
NIHAMB |
53.12 |
55.22 |
2.10 |
| 9 |
SAYE, GRACE R (10)
|
NIHHSC |
1:04.03 |
56.76 |
-7.27 |
| 10 |
SCIBETTA, MADELINE (10)
|
NITTSC |
58.01 |
1:01.35 |
3.34 |
|
WALTERS, ELIZABETH J (10)
|
NITWST |
48.08 |
43.62 |
-4.46 |
|
SCHAEFER, GRACE I (10)
|
NIEAST |
56.51 |
NS |
- |
|
CHRISTOPHER, HAILEY R (10)
|
NIGWST |
56.62 |
NS |
- |