| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Holzer, Avery G (6)
|
MAECCC |
29.56 |
24.41 |
-5.15 |
| 2 |
Aiken, Holly S (6)
|
MAUN |
27.86 |
25.64 |
-2.22 |
| 3 |
Schroeder, Caroline K (6)
|
MAUN |
29.33 |
30.51 |
1.18 |
| 4 |
Kelsey, Peyton M (5)
|
MAECCC |
|
32.46 |
- |
| 5 |
Chin, Sydney K (6)
|
MAECCC |
|
32.79 |
- |
| 6 |
Nehmad, Marcella B (6)
|
MASEA |
37.77 |
32.89 |
-4.88 |
| 7 |
Davidson, Taylor A (5)
|
MAECCC |
35.40 |
33.91 |
-1.49 |
| 8 |
Kowaleski, Calliope D (6)
|
MASEA |
|
34.58 |
- |
| 9 |
Kelly, Maggie G (5)
|
MABGH |
42.17 |
35.36 |
-6.81 |
| 10 |
Cassidy, Josie A (6)
|
MASEA |
53.56 |
36.29 |
-17.27 |
| 11 |
Wainwright, Ella G (5)
|
MAECCC |
|
45.73 |
- |
| 12 |
Scott, Irene G (6)
|
MAPVA |
50.17 |
46.09 |
-4.08 |
| 13 |
Bown, Zoey L (6)
|
MABGH |
39.15 |
49.95 |
10.80 |
| 14 |
Resnick, Reese B (5)
|
MAECCC |
|
56.78 |
- |
|
Reed, Julianna M (6)
|
MAECCC |
|
NS |
- |