| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Moldenhauer, Paige A (12)
|
NIEAST |
37.71 |
38.37 |
0.66 |
| 2 |
Daley, Lauren S (11)
|
NITWST |
38.69 |
39.36 |
0.67 |
| 3 |
Gawron, Samantha G (11)
|
NICLSC |
47.92 |
45.06 |
-2.86 |
| 4 |
Blake, Hannah G (11)
|
NISWAT |
50.92 |
45.30 |
-5.62 |
| 5 |
Sand, Makayla E (12)
|
NITWST |
50.08 |
45.73 |
-4.35 |
| 6 |
Skrzypczyk, Emily A (11)
|
NITWST |
46.83 |
46.27 |
-0.56 |
| 7 |
Larson, Lucia O (11)
|
NIEAST |
55.49 |
48.15 |
-7.34 |
| 8 |
Passarell, Charlotte O (12)
|
NITWST |
51.41 |
48.16 |
-3.25 |
| 9 |
Westbrook, Danika G (11)
|
NISWAT |
53.30 |
48.87 |
-4.43 |
| 10 |
Valentine, Megan E (11)
|
NIEAST |
57.47 |
49.00 |
-8.47 |
| 11 |
Wolfley, Eva M (11)
|
NICLSC |
|
49.41 |
- |
| 12 |
Umansky, Isabella C (11)
|
NIBAAC |
48.60 |
49.81 |
1.21 |
| 13 |
Murphy, Audrey L (12)
|
NIEAST |
|
50.22 |
- |
| 14 |
Goldman, Bailee M (11)
|
NICLSC |
|
50.70 |
- |
| 15 |
Canestrari, Willow G (11)
|
NITWST |
59.52 |
52.50 |
-7.02 |
| 16 |
Gascoyne, Louisa M (11)
|
NIEAST |
53.01 |
53.22 |
0.21 |
| 17 |
Burnett, Ella R (12)
|
NITWST |
1:02.26 |
54.16 |
-8.10 |
| 18 |
Casey, Taylor R (11)
|
NISWAT |
1:01.12 |
56.50 |
-4.62 |
|
Stoltman, Claire B (11)
|
NICLSC |
44.21 |
DQ |
- |