| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Spacciapolli, Victoria A (12)
|
NIHHSC |
29.28 |
29.04 |
-0.24 |
| 2 |
Bartlo, Anna (12)
|
NIGISC |
33.21 |
29.60 |
-3.61 |
| 3 |
Comstock, Rachel V (12)
|
NIHHSC |
31.72 |
32.14 |
0.42 |
| 4 |
Bean, Anna S (11)
|
NIEAST |
31.52 |
32.23 |
0.71 |
| 5 |
Kowell-Ure, Phoenix W (12)
|
NIEAST |
32.46 |
32.73 |
0.27 |
| 6 |
Patterson, Riley E (12)
|
NIGISC |
37.61 |
33.42 |
-4.19 |
| 7 |
Wdowiasz, Gracie J (11)
|
NIHHSC |
35.95 |
35.51 |
-0.44 |
| 8 |
Edwards, Adriana S (12)
|
NIGISC |
40.31 |
38.90 |
-1.41 |
| 9 |
DeCourcey, Miranda J (11)
|
NIGISC |
38.81 |
39.70 |
0.89 |
| 10 |
Burns, Kaitlyn J (11)
|
NIGISC |
45.88 |
48.89 |
3.01 |
| 11 |
Bauman, Cameryn (11)
|
NIEAST |
58.50 |
50.45 |
-8.05 |
|
Bentley, Abbie E (11)
|
NIGISC |
44.14 |
DQ |
- |