| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Phelps, Anna E (9)
|
MAUN-0 |
41.24 |
43.67 |
2.43 |
| 2 |
Collins, Ryleigh (9)
|
MAPFY |
46.22 |
45.92 |
-0.30 |
| 3 |
Zerfass, Ava D (9)
|
MAEMAC |
47.67 |
47.70 |
0.03 |
| 4 |
Iacobelli, Sophia C (9)
|
MAEMAC |
48.78 |
48.69 |
-0.09 |
| 5 |
Frank, Alice C (9)
|
MAEMAC |
52.16 |
49.60 |
-2.56 |
| 6 |
Lin, Simone (9)
|
MAPAAC |
52.50 |
50.41 |
-2.09 |
| 7 |
Tassinari, Marianne E (9)
|
MAPFY |
49.15 |
50.91 |
1.76 |
| 8 |
Lenett, Carly A (9)
|
MAEMAC |
|
53.85 |
- |
| 9 |
Wiragh, Kendall J (9)
|
MAEMAC |
54.09 |
54.07 |
-0.02 |
| 10 |
Settle, Ashley (9)
|
MAUNPA |
|
55.03 |
- |
| 11 |
Piacenti, Lily (9)
|
MAPFY |
|
57.42 |
- |
| 12 |
Karnhaukhova, Anastasiya (9)
|
MAPJA |
1:07.12 |
58.38 |
-8.74 |
|
Sadler, Cheyenne N (9)
|
MAUNAA |
54.01 |
DQ |
- |