| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Johnson, Madilyn M (14)
|
MAGAAC |
26.24 |
26.33 |
0.09 |
| 2 |
O'Malley, Lauren K (15)
|
MASPFD |
25.92 |
27.01 |
1.09 |
| 3 |
Robey, Layla A (14)
|
MANPAC |
27.26 |
27.19 |
-0.07 |
| 4 |
Murray, Amanda W (13)
|
MASPFD |
26.59 |
27.56 |
0.97 |
| 5 |
Poach, Sarah J (13)
|
MANPAC |
27.03 |
27.65 |
0.62 |
| 6 |
Walsh, Keira (13)
|
MAPENN |
27.62 |
28.15 |
0.53 |
| 7 |
Valbert, Emma B (14)
|
MAUN |
28.47 |
28.62 |
0.15 |
| 8 |
Steinmetz, Zoe N (14)
|
MANSC |
28.70 |
28.70 |
0.00 |
| 9 |
Bluestein, Talia R (16)
|
MANSC |
|
28.82 |
- |
| 10 |
Scanlon, Katie (13)
|
MASPFD |
28.93 |
29.14 |
0.21 |
| 11 |
Rothberg, Mina E (14)
|
MASPFD |
29.45 |
29.16 |
-0.29 |
| 12 |
Komorowski, Katie G (16)
|
MASPFD |
28.77 |
29.18 |
0.41 |
| 13 |
Schulz, Ashley D (13)
|
MASPFD |
29.73 |
29.37 |
-0.36 |
| 14 |
Manning, Jazziah A (13)
|
MAUN G |
|
29.50 |
- |
| 15 |
Kennedy, Jaelyn D (17)
|
MAUN |
28.59 |
29.54 |
0.95 |
| 16 |
Vangen, Ella (14)
|
MASPFD |
29.42 |
29.67 |
0.25 |
| 17 |
Olhasso, Miranda p (15)
|
MAPENN |
26.97 |
29.78 |
2.81 |
| 18 |
Forster, Delaney R (13)
|
MASPFD |
29.66 |
29.87 |
0.21 |
| 19 |
Wiley, Hannah E (15)
|
MAUN |
29.24 |
30.08 |
0.84 |
| 20 |
McCloskey, Reilly G (16)
|
MAPENN |
28.47 |
30.14 |
1.67 |
| 21 |
Harris, Elizabeth A (14)
|
MANSC |
29.12 |
30.15 |
1.03 |
| 22 |
Rutkowski, Bethany C (17)
|
UNAT |
29.11 |
31.10 |
1.99 |
| 23 |
Ruiz, Paullina (13)
|
MANSC |
33.48 |
33.81 |
0.33 |
| 24 |
Muchin, Mackenzie R (13)
|
MAPENN |
36.04 |
35.90 |
-0.14 |
|
Cosgrove, Scarlett J (13)
|
MAGAAC |
33.32 |
NS |
- |