| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Obuhanych, Zachary J (11)
|
NIFAST |
33.69 |
33.15 |
-0.54 |
| 2 |
Bodell, Jack W (13)
|
NIFAST |
45.00 |
40.01 |
-4.99 |
| 3 |
Fisk, Holly E (12)
|
NIFAST |
48.87 |
40.28 |
-8.59 |
| 4 |
Visconte, Alexandria L (12)
|
NIFAST |
40.35 |
40.65 |
0.30 |
| 5 |
Hellman, Aidan S (13)
|
NIFAST |
|
40.93 |
- |
| 6 |
Radell, Lauren M (12)
|
NIFAST |
43.34 |
44.94 |
1.60 |
| 7 |
Hagen, Nicolette E (11)
|
NIFAST |
45.12 |
46.35 |
1.23 |
| 8 |
Larzelere, Natalie E (9)
|
NIFAST |
48.98 |
50.08 |
1.10 |
| 9 |
Webb, Bryan D (11)
|
NIFAST |
51.67 |
50.69 |
-0.98 |
| 10 |
Nedelko, Gleb Y (13)
|
NIFAST |
|
50.82 |
- |
| 11 |
Lindquist, Matthew J (11)
|
NIFAST |
55.28 |
55.15 |
-0.13 |
| 12 |
Preston, Evan W (10)
|
NIFAST |
58.69 |
55.97 |
-2.72 |
| 13 |
Montaque, Angelina M (10)
|
NIFAST |
55.80 |
56.78 |
0.98 |
| 14 |
Huerta-Andrade, Amanda (9)
|
NIFAST |
|
1:08.51 |
- |
|
Togay, Eyup A (12)
|
NIFAST |
1:18.71 |
DQ |
- |
|
Black, Kaitlin (12)
|
NIFAST |
|
NS |
- |