| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Crye, Jessie R (8)
|
NIFAST |
40.87 |
42.35 |
1.48 |
| 2 |
Weiner, Isabella G (9)
|
NIFAST |
48.68 |
46.53 |
-2.15 |
| 3 |
Preston, Evan W (10)
|
NIFAST |
52.15 |
47.20 |
-4.95 |
| 4 |
Eggar, Renee E (10)
|
NIFAST |
|
49.73 |
- |
| 5 |
Vadney, Sage E (11)
|
NIFAST |
52.30 |
50.38 |
-1.92 |
| 6 |
Lawrence, Taylor B (11)
|
NIFAST |
51.38 |
51.92 |
0.54 |
| 7 |
Montaque, Angelina M (10)
|
NIFAST |
58.71 |
52.46 |
-6.25 |
| 8 |
Sanders, Nina P (9)
|
NIFAST |
|
52.64 |
- |
| 9 |
Sherman, Ani E (10)
|
NIFAST |
52.03 |
53.07 |
1.04 |
| 10 |
Forsgren, Emma J (11)
|
NIFAST |
53.73 |
54.93 |
1.20 |
| 11 |
Claus, Alivia M (13)
|
NIFAST |
58.51 |
54.95 |
-3.56 |
| 12 |
Huerta-Andrade, Amanda (9)
|
NIFAST |
57.67 |
56.69 |
-0.98 |
| 13 |
Togay, Erdem A (9)
|
NIFAST |
1:15.50 |
1:01.26 |
-14.24 |
| 14 |
Williams, Gavin S (9)
|
NIFAST |
|
1:05.71 |
- |
| 15 |
Boyum, Paige L (8)
|
NIFAST |
|
1:09.87 |
- |
|
Comstra, Connor D (7)
|
NIFAST |
1:00.93 |
DQ |
- |
|
Brett, Erin E (9)
|
NIFAST |
49.08 |
DQ |
- |