| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Sikes, Zade A (16)
|
NTMTRO |
24.49 |
23.86 |
-0.63 |
| 2 |
Stangl, Donovan S (16)
|
NTMTRO |
24.44 |
25.33 |
0.89 |
| 3 |
Peterson, Jared P (15)
|
NTMTRO |
25.49 |
25.54 |
0.05 |
| 4 |
Bervig, Colton A (16)
|
NTMTRO |
|
25.67 |
- |
| 5 |
Poulton, Andrew M (15)
|
NTMTRO |
26.00 |
25.93 |
-0.07 |
| 6 |
Hodgkins, Dennis C (15)
|
NTMTRO |
25.72 |
26.42 |
0.70 |
| 7 |
Morgan, Conner D (15)
|
NTMTRO |
26.64 |
27.15 |
0.51 |
| 8 |
Shalaby, Abdelrahman K (15)
|
NTMTRO |
27.50 |
28.13 |
0.63 |
| 9 |
Liu, Eric R (15)
|
NTMTRO |
28.00 |
28.20 |
0.20 |
| 10 |
Reagan, Sam A (16)
|
NTMTRO |
|
28.30 |
- |
| 11 |
Go, Alexander Q (15)
|
NTMTRO |
28.54 |
28.39 |
-0.15 |
| 12 |
Fernaine, Michael J (15)
|
NTMTRO |
|
28.52 |
- |
| 13 |
Trivedi, Arjun H (15)
|
NTMTRO |
29.94 |
29.27 |
-0.67 |
| 14 |
Medina, Logan J (17)
|
NTMTRO |
36.54 |
30.18 |
-6.36 |
| 15 |
Waller, Christopher A (21)
|
NTMTRO |
34.12 |
35.52 |
1.40 |
|
Caretti, Bruno (17)
|
NTMTRO |
28.16 |
NS |
- |