| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Juskalian, Tyler J (15)
|
MAPCAC |
50.72 |
50.71 |
-0.01 |
| 2 |
Kasznay, Jonah (16)
|
MAUN-L |
51.96 |
51.83 |
-0.13 |
| 3 |
McIlroy, Aidan C (15)
|
MAUN-L |
52.75 |
51.92 |
-0.83 |
| 4 |
Anderson, Zach N (17)
|
MARAYS |
53.11 |
52.96 |
-0.15 |
| 5 |
Clark, John F (15)
|
MAGAAC |
54.07 |
53.06 |
-1.01 |
| 6 |
Lemishko, Michael A (15)
|
MAPCAC |
50.43 |
53.24 |
2.81 |
| 7 |
McIver-Jenkins, Benjamin A (15)
|
MASERP |
55.97 |
55.03 |
-0.94 |
| 8 |
Roling, Simon G (15)
|
MAPCAC |
56.47 |
55.45 |
-1.02 |
| 9 |
Tuffner, Cole J (16)
|
MALCY |
55.79 |
55.60 |
-0.19 |
| 10 |
Marcinkowski, William (17)
|
MACHEL |
1:01.24 |
57.79 |
-3.45 |
| 11 |
Clark, Evan J (15)
|
MARAYS |
58.29 |
58.22 |
-0.07 |
| 12 |
Cohn, Josh B (15)
|
MAACAC |
1:01.57 |
1:03.48 |
1.91 |
| 13 |
Rankin, Connor E (16)
|
MALYM |
1:25.21 |
1:19.37 |
-5.84 |
|
Mortagy, Amr K (18)
|
MAGAAC |
50.77 |
NS |
- |
|
Inman, Jack C (17)
|
MALCY |
50.60 |
NS |
- |
|
Dencker, Jean-Paul (18)
|
MAPCAC |
49.92 |
NS |
- |
|
Esslinger, Robert E (17)
|
MAUN-L |
50.33 |
NS |
- |