| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Trumpower, Adayaleise L (7)
|
OHUN |
21.29 |
19.80 |
-1.49 |
| 2 |
Burkey, Brynn M (8)
|
OHFMST |
21.58 |
20.44 |
-1.14 |
| 3 |
Stelitano, Mia S (8)
|
AMKW |
24.53 |
20.78 |
-3.75 |
| 4 |
Stirm, Avary R (7)
|
LECCST |
21.79 |
21.76 |
-0.03 |
| 5 |
Maddox, Reagan J (8)
|
AMANSC |
23.59 |
22.26 |
-1.33 |
| 6 |
Ukrayinets, Anastasiya (8)
|
AMANSC |
23.76 |
22.59 |
-1.17 |
| 6 |
Stannert, Lillian G (8)
|
OHFMST |
21.58 |
22.59 |
1.01 |
| 8 |
Ukrayinets, Sofiya (8)
|
AMANSC |
25.37 |
22.79 |
-2.58 |
| 9 |
Sunday, Alaina J (7)
|
OHFMST |
23.76 |
23.25 |
-0.51 |
| 10 |
Rando, Maylie P (8)
|
OHSOS |
26.26 |
24.02 |
-2.24 |
| 11 |
Clifford, Hazel A (8)
|
AMKW |
25.34 |
24.29 |
-1.05 |
| 12 |
Lopez, Jennifer * (8)
|
OHBGSC |
23.86 |
24.52 |
0.66 |
| 13 |
Kin, Emily L (7)
|
OHFAST |
23.85 |
24.55 |
0.70 |
| 14 |
Laman, Elizabeth P (8)
|
AMKW |
23.90 |
24.68 |
0.78 |
| 15 |
Sunderlin, Charlotte R (8)
|
AMANSC |
29.43 |
24.73 |
-4.70 |
| 16 |
Lim, Sharon Y (7)
|
OHSYL |
27.97 |
24.92 |
-3.05 |
| 17 |
Agens, Gemma M (8)
|
AMKW |
30.19 |
24.98 |
-5.21 |
| 18 |
Wittmer, Marin A (7)
|
OHFMST |
22.21 |
25.02 |
2.81 |
| 19 |
Rhoades, Emilia M (8)
|
OHSOS |
24.53 |
25.19 |
0.66 |
| 20 |
Constable, Aubrey C (8)
|
AMANSC |
24.54 |
25.23 |
0.69 |
| 21 |
Eisenhauer, Brooklynn G (7)
|
OHSOS |
|
26.12 |
- |
| 22 |
Hollenbeck, Mackenzie E (7)
|
AMANSC |
27.55 |
26.45 |
-1.10 |
| 23 |
Coffman, Margaret J (7)
|
AMANSC |
27.06 |
26.57 |
-0.49 |
| 24 |
Hatt, Chloe K (7)
|
LEPCP |
30.01 |
26.70 |
-3.31 |
| 25 |
Thomas, Lucy J (8)
|
OHUN |
26.16 |
27.20 |
1.04 |
| 26 |
Glass, Callie E (6)
|
AMANSC |
27.35 |
28.83 |
1.48 |
| 27 |
Eaglen, Lakelyn E (6)
|
OHSOS |
|
53.86 |
- |
|
O'Toole, Delaney S (8)
|
AMKW |
16.78 |
NS |
- |
|
Alt, Eden E (8)
|
OHBGSC |
|
NS |
- |
|
Carpenter, Susan G (8)
|
OHSYL |
28.72 |
NS |
- |