| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Arreguin, Ayleen (11)
|
GUHCAP |
30.83 |
30.42 |
-0.41 |
| 2 |
Pendle, Addisyn T (12)
|
GUSHRK |
32.71 |
31.15 |
-1.56 |
| 3 |
Cousin, Amber J (11)
|
GUHCAP |
31.27 |
31.30 |
0.03 |
| 4 |
Gard, Rebecca A (11)
|
GUSSS |
36.16 |
32.84 |
-3.32 |
| 5 |
Esbeck, Olivia J (11)
|
GUSHRK |
32.34 |
32.90 |
0.56 |
| 6 |
York, Peyton B (11)
|
GUHCAP |
34.97 |
33.33 |
-1.64 |
| 7 |
Koncaba, Alice J (11)
|
GUSHRK |
34.40 |
33.56 |
-0.84 |
| 8 |
Parkhurst, Azalea L (12)
|
GUSHRK |
40.19 |
34.32 |
-5.87 |
| 9 |
Woodby, Addison N (11)
|
GUAQUA |
34.35 |
34.90 |
0.55 |
| 10 |
Sustala, Delaney (11)
|
GUSSS |
35.24 |
35.05 |
-0.19 |
| 11 |
Migl, Lilly J (11)
|
GUSHRK |
35.23 |
35.16 |
-0.07 |
| 12 |
Patel, Diya R (11)
|
GUSSS |
38.59 |
37.15 |
-1.44 |
| 13 |
Rajszewski, Magda (11)
|
GUHCAP |
|
37.32 |
- |
| 14 |
Patel, Bhavini G (12)
|
GUSSS |
|
37.38 |
- |
| 15 |
Pinkoski, Pallas N (11)
|
GUSHRK |
38.74 |
37.87 |
-0.87 |
| 16 |
Medellin, Mackenzie K (11)
|
GUSSS |
34.82 |
38.83 |
4.01 |
| 17 |
Castro, Miaangelina (11)
|
GUHCAP |
|
39.80 |
- |
| 18 |
Luong, Katie M (12)
|
GUAQUA |
|
40.26 |
- |
| 19 |
Patel, Diya J (11)
|
GUSSS |
|
40.33 |
- |
| 20 |
Klesel, Rebekah B (12)
|
GUSHRK |
40.60 |
40.68 |
0.08 |
| 21 |
Rupp, Julia C (11)
|
GUSHRK |
42.71 |
40.90 |
-1.81 |
| 22 |
Allis, Emma I (11)
|
GUSHRK |
41.14 |
44.60 |
3.46 |
|
Serna-Cohen, Rachel J (11)
|
GUCOOG |
|
DQ |
- |
|
Fotso, Price LaLande K (12)
|
GUHCAP |
33.00 |
NS |
- |
|
Acevedo, Daniela E (11)
|
GUHCAP |
33.69 |
NS |
- |